The Preparation Of Metal Nanocluster Complex Fluorescent Probe And Its Application In Drug Detection

Metal nanoclusters as a new type of metallic fluorescent nanomaterial composed of several to several hundreds of metal atoms.Due to their unique physical and chemical properties,metallic nanoclusters have attracted wide attention in the academic community.Metal nanoclusters have many advantages,such as strong photoluminescence,good quantum yield,large Stokes shift,adjustable fluorescence emission,strong fluorescence stability,simple synthesis methods and biocompatibility,etc.,and have been developed to a promising fluorescent probe in the field of biosensing and imaging.Our study is designing biosensor for biomolecules detection based on fluorescence properties of metal nanoclusters,three new detection systems was designed using metal nanocluster composite as fluorescent probes.The main research content is as follows:（1）Using N₂H₄·H₂O as reducing agent and bovine serum albumin（BSA）as stabilizer,a red fluorescence emitting copper nanoclusters（CuNCs）were prepared in bovine serum albumin（BSA）matrix.When adding rutin the rutin can interact with CuNCs by hydrogen bond and fluorescence quenching was occurred due to the electron transfer from CuNCs to Rutin.Under the optimal conditions,the fluorescence intensity of CuNCs decreases linearly with increasing rutin concentration,with a linear range of0.1-100μM and detection limit of 0.02μM.Moreover,the FL quenching response of CuNCs is highly selective and sensitive to rutin in real human serum samples,Importantly,rutin-based fluorescent probes can perform rutin FL sensing and rutin visualization FL detection in aqueous solution and on filter paper.（2）A dual-emission ratio fluorescence probe based on organic dye fluorescein and CuNCs was designed.Through polyethylene amine（PEI）,the red fluorescence emission CuNCs is connected with the green fluorescence emission fluorescence,and the prepared fluorescent/CuNCs composite system is pink fluorescence emission.When morpholinium hydrochloride was added,the color of the composite system is changed from pink to green by electrostatic interaction between positively-charged guanidine and copper nanoparticles.The linear detection range of Mor is 0-2.5μM,and the detection limit is0.26μM.The results showed that the detection of the dual-emission fluorescence probe showed excellent detection capability in real samples,and it can realized the visualization detection of Mor in aqueous solution and filter paper.（3）Blue-emitting silicon quantum dots（SiNPs）and red-emitting gold nanoclusters（AuNCs）were successfully prepared.Through EDC/NHS activation and coupling,a novel functional nanocomposite composed of SiNPs and AuNCs was prepared,which showed dual fluorescence emission.When carbimazole（CBZ）was added,the fluorescence intensity of AuNCs significantly decreased at 666 nm,which was due to the electron transfer between the conjugated structure in CBZ and AuNCs,however,the fluorescence intensity of AuNCs at 450 nm is almost constant.Based on the above,a new ratio fluorescence probe system was prepared for CBZ detection.Under optimal experimental conditions,the linear detection range is 0-5μM and the detection limit is0.02μM,achieving sensitive and selective detection of CBZ.