Design, Preparation And Performance Study Of Multifunctional Nano-fluorocarbon Fiber-based Anti-cancer Drug Carrier

In recent years,carbon materials such as fullerenes,carbon nanotubes and graphene have been widely studied in the biological fields due to their unique physicochemical properties,however,fluorinated carbon fibers(FC)have been seldom explored in biomedicine because of their large-size,needle-like structure and strong hydrophobicity.In this project,we first developed a novel FC-based nanocarrier with good biocompatibility,high drug loading and excellent photothermal conversion performance.We also studied the preparation,characterization of functionalized FC and explored their performance in cancer treatment.First,a simple and effective strategy was adopted to convert hydrophobic FC into fluorinated carbon fiber oxide(FCO)with nano-sized structure and good water solubility in both water and medium by a designed hydroxyl substitution and acid oxidation reaction,and we researched their elemental composition,structure,morphology.It was proved that FCO had a large surface area,and they also retained a large amount of fluorine element while introducing rich oxygen-containing functional group.Meanwhile,their hydrophilicity and water solubility were significantly enhanced.The conjugatedπorbitals from the sp~2 hybrid carbon also endowed them with excellent photothermal conversion properties,the experimental results showed that FCO with a concentration of 140μg/mL could be heated up to 50 ~oC within 600 s when exposed to the near-infrared laser(NIR)with 1.8 W/cm~2.We further investigated FCO loaded anticancer drug doxorubicin(DOX)and the DOX release behavior under different pH values in vitro.Experiments showed that drug release performance exhibited typical pH-responsive behavior and a certain degree of sustained release effect.Biological experiments showed that the constructed nanocarriers exhibited excellent biosafety and readily entered cells via endocytosis.At the same time,the effective combination of chemotherapy and hyperthermia showed more excellent therapeutic effects on tumors.Considering the low cost,high synthesis efficiency and excellent performance,newly synthesized FCO had a great research value as a novel drug nano-carrier.The targeted delivery enhanced the anti-tumor effect of chemotherapeutic drugs while reducing side effects on normal cells and tissues.We have synthesized FA-BSA/FCO/Ag composites by modifying folate molecules(FA)linked bovine serum albumin(BSA)and silver nanoparticles(Ag)on the surface of FCO,and studied the composition,structure,morphology,photothermal properties and so on.In addition,FA-BSA/FCO/Ag+DOX were demonstrated to have a sustained-release and pH-responsive release behavior by studying drug loading and release behavior.Biological experiments showed that FA-BSA/FCO/Ag+DOX could specifically bind with the FA receptor on the surface of He La cells.Therefore,the toxicity to HeLa cells was significantly higher than that of A549 cells without FA receptor,and the cytotoxicity was significantly enhanced under the irradiation of NIR laser.In general,FA-BSA/FCO/Ag+DOX could enhance the therapeutic effect of anticancer drugs,while the effective combination of chemotherapy and hyperthermia showed a greater effect on tumor treatment,which proved great potential of their application in the biomedical field.