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The Effect Of Noise On The Anxiety Behavior Of Black-lined Hairy Foot Rats And Its Molecular Mechanism

Posted on:2020-04-25Degree:MasterType:Thesis
Country:ChinaCandidate:Y Z ChenFull Text:PDF
GTID:2430330578956892Subject:Genetics
Abstract/Summary:PDF Full Text Request
As an environmental stimulus,highway noise can activate the HPA axis.The CRHR1 receptor plays a key role in the response of the HPA axis to external stimuli.We explored the effects of acute and chronic noise on Phodopus sungorus behavior and CRHR1 expression in the hypothalamus.40 adult Phodopus sungorus were randomly divided into the following 8 groups,5 in each group:(1)F AC,Female Acute Control,(2)F A85,Female Acute 85 dBA exposure;(3)M AC,Male Acute Control,(4)M A85,Male Acute 85 dBA exposure;(5)F CC,Female Chronic Control,(6)F C85,Female Chronic 85 dBA exposure;(7)M CC,Male Chronic Control,(8)M C85,Male Chronic 85 dBA exposure.The acute noise treatment group was treated with 85 dBA highway noise for 4h,and the chronic noise treatment group was used for 4 hours per day for the same noise condition for 30 days.CRHR1 in the hypothalamus was detected using realtime fluorescence quantification.The behavioral test include the open field test,the light and dark box test,and the elevated plus maze.The behavioral experiment was performed only once after acute noise treatment,and behavioral experiment was performed every 5 days after chronic noise treatment.Acute noise caused slight anxiety behavior in male and female Phodopus sungorus,and had no significant effect on the expression of CRHR1 in the hypothalamus.Chronic noise can induce abnormal impulsive and hyperactive low anxiety behavior in males and significantly reduce the expression of CRHR1 in hypothalamus.Chronic noise made females also producing low anxiety behavior,but the effect of noise on its behavior was less than that of males.There was no significant difference in CRHR1 expression in chronic female hypothalamus.Correlation analysis showed that the expression of CRHR1 in the hypothalamus was extremely significant negative correlated with the movement time in central area r=-0.579,P<0.01,and was significant negative correlated with the movement distance in central area r=-0.466,P=0.014,but not significantly correlated with other behavioral indicators.This study has shown that acute and chronic noise can lead to behavioral changes in Phodopus sungorus,and chronic noise can lead to the decrease of CRHR1 expression in the hypothalamus of male individuals.This study provides a theoretical basis for mastering the role of CRHR1 in noise-regulated animal behavior.
Keywords/Search Tags:Phodopus sungorus, Highway noise, CRHR1, Anxious behavior, HPA axis
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