EQTL Studies The Regulatory Network Of Mosquito Resistance Genes

Mosquitoes are important medical arthropods,which transmit a variety of mosquito-borne pathogens.Several outbreaks of mosquito-borne diseases have been harmful to human health,is an important global public health problem.The chemical control of mosquitoes,via the use of insecticides,is the primary means of managing the spread of these diseases.However,the widespread and improper use of insecticide has resulted in the evolution of resistance,which has become a major obstacle for mosquito-borne diseases management and exacerbated the spread of mosquito-bome disease.Mosquito insecticide resistance is actually a complex phenotype of polygenic inheritance.Early researchers used a variety of methods to investigating the mechanisms of insecticide resistance?Indeed,none of the currently known genes can entirely explain the molecular basisof insecticide resistance.Expression Quantitative Trait Loci,eQTL,regard mRNA expression level of each gene as a quantitative trait,and performed QTL analysis with gene polymorphism data.It can analyze regulatory relationships and synergistic effect between genes,looking for upstream regulatory sites and downstream regulated genes.Eventual establishment the regulatory networks of genes,clarifying the mechanism of gene regulation.In this study,we used eQTL to research deltamethrin resistance associated gene of Culex pipiens pallens for the first time.In order to establish regulatory networks and look for major resistance gene.We developed the crosses of the insecticide-susceptible strain(S)and deltamethrin-resistant strain(R)with Culex pipiens pallens and get F2 intercross segregating populations.Then qualified the F2 hybrids into 4 kinds of phenotype according to WHO adult bioassay.We used De nove transcriptome sequencing(RNA-seq)and analyzed by bioinformatics.In this experiment we chose 1263 DEGs and 42,196 SNP loci data for downstream analysis,obtain 98 cis-eQTL SNPs and 11046 trans-eQTL SNPs.The main biological functions are female meiosis chromosome segregation?actin binding?calcium ion binding?integral to membrane.According to the analysis of trans-eQTL gene regulation relationship,we used Cytoscape to draw regulation of gene expressing network diagram,initially established a resistance gene regulatory networks.From the resistance gene regulatory networks,we find CL9880.CT1_A may be a major gene,which functions are mainly cellular amino acid metabolic process,asparaginase activity,lysophospholipase activity.In this study,the resistance difference between male and female mosquitoes were analyzed,obtain 5 cis-eQTL SNPs and 517 trans-eQTL SNPs,which have a strong relationship with Gender characterized.The main biological functions are locomotor rhythm?glutamate catabolic process to 2-oxoglutarate?glutamate dehydrogenase[NAD(P)+]activity?mitochondrial matrix.This study provides information for in-depth study of mosquito-borne resistance mechanisms and theoretical basis for resistance management and the development of new highly effective pesticides.