Numerical Simulation And Experimental Study On Driving And Mixing Of Microfluidic Chip Based On PCB

With the continuous advancement of science and technology,traditional analytical testing equipment has been unable to meet the needs of many fields.In order to meet various testing needs,modern analytical testing technology is constantly developing towards miniaturization,portability and intelligence.The microfluidic chip just meets the needs of this development direction.Microfluidic chip is a new technology platform that integrates transportation,mixing and separation processes in the fields of biology and chemistry into microchannels.It has good application prospects in the fields of biology,medicine and chemistry.In this paper,the microfluidic chip system will be studied from the two aspects of solution transport and solution mixing,and they will be combined to design a microfluidic chip system that can independently drive and mix the solution by surface tension.The research content of the thesis mainly includes the following aspects:1.By studying the basic principle and control methods of surface tension,the preliminary design of the channel width and height of the microfluidic chip and the angle between the inlet channels were carried out.The self-driving of surface tension was numerically simulated to explore the feasibility of surface tension as the driving force of microfluidic chip.By modifying the surface of comparative experiments,T-channel self-driven theory and simulation experiments to verify the reliability,and to obtain a more favorable result.2.By studying the theory of induced electroosmotic flow generation,the numerical simulation of the symmetric vortex theory and mixing effect of induced electroosmotic flow is carried out to explore the feasibility of inducing electroosmotic flow as a microfluidic chip mixer.By vortex mixing experiments and theoretical results show the reliability theory and simulation.Based on the experimental results,the microfluidic chip was improved,and the preparation of the mixer based on PCB board was proposed.3.Numerical simulation of the chip design obtained in the previous section,construction of an experimental platform,fabrication of integrated chips that can be self-driven by surface tension and induced electroosmotic flow mixing.Observe the driving effect by using a microscope,and further optimize the chip based on the actual results obtained.By loading OpenCV,writing a Python program to extract and process image data,and calculating its uniformity analysis mixing efficiency.This research can help microfluidic chip design.