Construction Of The Full-field Optical Coherence Tomography And High-quality Noninvasive Research Of The Early Embryonic Morphologic Image

In this paper,the full-field optical coherence tomography(OCT)was used as the research object and a full-field OCT system was set up to obtain high-quality tomographic images of biological samples.The full-field OCT has the characteristics of no damage,high resolution and fast imaging and it has broad prospects in the field of biomedicine.The full-field OCT is the best technology to study the morphological development of the early embryo,while the full-field OCT images are hard to avoid noises.Due to the noises,the boundaries between the different organizations in the early embryo are ambiguous,which would cause interference of organization segmentation.To solve this problem,this paper processed full-field OCT images of early embryos with the median filter,wiener filter and anisotropic diffusion algorithm,and evaluate the noise reduction effect using the signal-to-noise ratio,the mean square error,peak signal to noise ratio and edge retention index.It was shown that early embryo images after noise reduction with anisotropic diffusion algorithm kept rather complete original information and have rather clear boundaries,the visual effect of which is the best.It would be come true that the reproductive development problem could be study by the full-field OCT system combined with the culture technology in-vitro.In-vitro culture is a very important technical tool in clinical and basic research.During the process of in-vitro extraction,oocytes or early embryos can't avoid the visible light stimulation.However,the effects of light exposure on the development quality in-vitro are still unclear.This work used three technologies of in-vitro culture,noninvasive imaging(full-field OCT)and three-dimensional reconstruction.Under the prerequisite of ensuring the same light intensity and the same exposure time,we were the first to use the real-time and in-situ method to study the effects of different visible spectra on the development quality of mouse oocytes and embryos based on the morphology.It was found that(1)compared with the white light illumination,the blue-violet light significantly reduced the rates of the germinal vesicle breakdown and the first polar body extrusion of mouse oocytes,while under the green and red light illumination,these two rates greatly increased.(2)compared with the white light group,the blastocyst rate reduced and apoptosis increased greatly in the blue-violet light group,while the opposite was true for the green and red light group.It indicated that blue-violet light was harmful to the development of mouse oocytes and early embryos,while the green light and red light showed little damage.The results of the study have important implications for germ cell development under in-vitro conditions.