Intervention Of Oyster Peptide On Mineral Metabolism In Rats After Exhaustive Swimming Training

Objectives:To explore the effects of 6-week exhaustive swimming training on mineral metabolism in rats and the intervention of oyster peptide,so as to provide experimental basis for the specific application of oyster peptide in sports practice.Method:Forty healthy male Wistar rats,aged 6 weeks and weighing 210± 10 g,were randomly divided into four groups:control group(NC,n=10),oyster peptide group(OP,n=10),exercise group(E,n=10)and exercise+oyster peptide group(OPE,n=10).All rats were fed and fed freely(deionized water).Incremental negative gravity exhaustive swimming was used in group E and OPE 6 times a week.Gastric oyster peptide(2.Og/kg)was administered in OP and OPE groups at a fixed time and 30 minutes after each training,respectively.Six weeks later,the contents of zinc(Zn),iron(Fe),calcium(Ca)and magnesium(Mg)in feed,feces,serum and liver were measured,and the apparent absorption rate of minerals was calculated.At the same time,the contents of ferritin(SF),transferrin(TRF)and Hepcidin(Hepc)in serum were measured.Results:(1)Bivariate analysis of variance showed that supplementation of oyster peptide and exhaustive swimming training had no significant interaction with serum iron,calcium,magnesium,ferritin and transferrin contents in rats(P>0.05);exhaustive swimming training could reduce serum iron and magnesium contents in rats,but there was no significant difference;while serum calcium,ferritin and transferrin contents were significant.The levels of serum iron(P<0.05),ferritin(P<0.05)and transferrin(P<0.01)were significantly increased by oyster peptide supplementation.There was significant interaction between oyster peptide supplementation and exhaustive swimming training(P<0.05).Compared with NC group,serum zinc in group E decreased significantly(P=0.005,P<0.01),but there was no significant difference in serum zinc between OPE group and OPE group(P=0.961,P>0.05).(2)Bivariate analysis of variance showed that supplementation of oyster peptide had significant interaction in reducing iron content in liver of exhaustive exercise rats(P<0.05);exhaustive swimming training could significantly increase iron content in liver of rats(P<0.01),while supplementation of oyster peptide had significant effect on reducing iron content in liver of rats(P<0.05).(3)Bivariate analysis of variance showed that supplementation of oyster peptide had no significant interaction with increasing intestinal pH,serum Hepcidin content and apparent iron absorption rate in exhaustive swimming training rats.Exhaustive swimming training could significantly increase serum Hepcidin content and decrease apparent iron absorption rate(P<0.05),while supplementary oyster peptide significantly decreased serum Hepcidin content,intestinal pH and apparent iron absorption(P<0.05)..Conclusion:(1)Long-term exhaustive swimming training resulted in disorders of iron and zinc metabolism in rats,and oyster peptide supplementation could improve this.(2)Long-term exhaustive swimming training can not only increase iron deposition in liver,but also increase the secretion of Hepcidin,which can reduce the absorption of iron and other minerals in intestinal tract.Supplementation of oyster peptide after exhaustive swimming can reduce the pH value of cecum on the one hand,and inhibit the secretion of Hepcidin,thus effectively increase the absorption of iron and other minerals in intestinal tract.