Expression,Function And Clinical Significance Of LncRNA SMAD5-AS1 In Nasopharyngeal Carcinoma

Objective: Analyze the levels of LncRNA SMAD5-AS1 expression in nasopharyngeal carcinoma tissues and cells;Observe the effect of its expression on the proliferation,migration and apoptosis of nasopharyngeal carcinoma;Study the relationship between the expression of LncRNA SMAD5-AS1 and the clinicopathological characteristics and prognosis of patients.Methods :Analyze the different expression of LncRNA SMAD5-AS1 in nasopharyngeal carcinoma expression chips GSE53819 and GSE64634 in Gene Expression Omnibus data(https://www.ncbi.nlm.nih.gov/geo/).The “limma” package programmed in R language is used to analyze the chips and “phymap” is used to construct a heat map of differently expressed genes.Venn Chart Software is to Get Venn(http://bioinformatics.psb.ugent.ugent.be/webtools/Venn/).LncRNA SMAD5-AS1 was selected for different expression levels between nasopharyngeal carcinoma tissues and normal nasopharyngeal epithelium.The detection of SMAD5-AS1 expression in nasopharyngeal carcinoma tissue samples and adjacent tissues is through rt-PCR,and the basic information andclinical data of the patients are collected,and lastly we analyze the relationship between the expression of LncRNA SMAD5-AS1 and the clinical prognosis of patients with nasopharyngeal carcinoma.To explore the effects of LncRNA SMAD5-AS1 expression on proliferation,apoptosis,invasion and migration of nasopharyngeal carcinoma cells,we use Ed U method and flow cytometry to detect the proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cells,and we use transwell method to detect cell invasion and migration of nasopharyngeal carcinoma cells.Stabilized transfected CNE-2 cells are injected into nude mice to observe the growth changes of nasopharyngeal carcinoma cells in sh-SMAD5-AS1 groups and sh-NC groups,respectively.In this way,we explore the effect of LncRNA SMAD5-AS1 silencing on the growth of nasopharyngeal carcinoma cells.Results: The tissues and cells of nasopharyngeal carcinoma showed higher LncRNA SMAD5-AS1 expression levels than control group(P <0.0001,P <0.0001),and LncRNA SMAD5-AS1 silencing inhibits the growth,proliferation,invasion and migration of nasopharyngeal carcinoma cell(P <0.05,P <0.0001,P <0.0001,P <0.0001),but on the other hand,it promotes apoptosis in nasopharyngeal carcinoma cells(P<0.0001).Upregulation of LncRNA SMAD5-AS1 expression positively associates with T stage,N stage,and TNM stage of nasopharyngeal carcinoma,tumor-related deaths,treatment of distant metastases(P =0.003,P=0.018,P =0.014,P =0.009,P =0.006).The expression of LncRNA SMAD5-AS1 was negatively correlated with patients' OS?PFS and DMFS(P=0.001,P =0.012,P =0.001).Conclusions:Nasopharyngeal carcinoma tissues and cells showed higher levels of LncRNA SMAD5-AS1 compared with thecontrol group,which promotes growth,proliferation,invasion and migration of nasopharyngeal carcinoma cells but inhibits apoptosis.2.Upregulated LncRNA SMAD5-AS1 expression was positively correlated with T-stage,N-stage and TNM stage,tumor-associated death,post-treatment distant metastasis.Up-regulation of LncRNA SMAD5-AS1 reduced OS,PFS and DMFS of NPC patients.Hence,LncRNA SMAD5-AS1 may be a potential therapeutic target for NPC intervention.