Influence Of Low-level Laser Irradiation Via Wnt/?-Catenin Signal Pathway On RKO Colorectal Cancer Cells

Normal expression of the Wnt/?-catenin signal pathway plays an important role in regulation of normal cell growth and differentiation,and research on target sites in the Wnt/?-catenin signal pathway thus has become a hotspot on targeted anticancer therapy.Low-level laser irradiation(LLLI)was discussed for its influence on RKO colorectal cancer cells through the Wnt/?-catenin signal pathway.RKO cells were irradiated by the 632.8 nm infrared laser,and RT-PCR method was adopted for screening gene expressions of APC??-catenin and DVL in the Wnt/?-catenin signal pathway of RKO cells irradiated by a gradient low-level laser.Results of the RT-PCR show that the low-level laser with an energy density of 15 J/cm~2reduced?-catenin expression of RKO cells,and increased APC and DVL expressions,indicating both an increasing trend as the rising energy density and a statistical discrepancy.Related indicators of RKO cells were tested for their changes by q RT-PCR and Western blot methods,under the low-level laser irradiation of 15 J/cm~2.Results of q RT-PCR show that gene expressions of Wnt5A?RPL5 and TCF-4 indicated different levels of reduction,with a statistical discrepancy.Results of Western blot show that reduction in protein expression of?-catenin,relative to that of the blank control group,had a statistically significant discrepancy.Both q RT-PCR and Western blot show that low-level laser irradiation of15 J/cm~2 for short time still had an inhibiting effect on overexpression of Wnt/?-catenin signal pathway of RKO cells.By observation on low-level laser irradiation of 15 J/cm~2through viable and dead-cell kits staining,it can be seen that the dead RKO cells in experimental group were more than that in control group.In conclusion,low-level laser irradiation of 15 J/cm~2can delay proliferation of RKO colorectal cancer cells through inhibition on overexpression of Wnt/?-catenin signal pathway,and is dose-dependent with a time-accumulation effect;it can also activate the apoptosis pathway and therefore incur cell apoptosis.This paper,in view of the above,provided a new thinking for treatment of colorectal cancer.