| Objective:1.To evaluate the curative effect of Shufeng Zhijing Recipe in treating children’s epilepsy,and to discuss the rule of Professor Ma Rong’s application of Shufeng Zhijing Recipe in treating children’s epilepsy.2.To preliminary explore the effect of Shufeng Zhijing Recipe on seizures and the concentration of antiepileptic drug carbamazepine in cerebrospinal fluid in drug-resistant epilepsy model rats.Methods:1.Using retrospective research methods,62 cases of epilepsy children treated by Professor Ma Rong with Shufeng Zhijing Recipe were collected,and their gender,age of onset,seizure performance,seizure frequency,seizure inducement,past history,family history,brain Electrogram and MR,the situation of taking anti-epileptic western medicine and the characteristics of traditional Chinese medicine syndromes,etc.,use the Excel table to establish the data bank to store all relevant information,and use SPSS20.0 statistical software for statistical analysis.Evaluate the curative effect of Shufeng Zhijing Decoction on children with epilepsy,and discuss its clinical application.2.Select 100 rats and randomly select 10 rats as the normal group,and the remaining 90 rats are ignited by lithium chloride-pilocapine model of epilepsy rat model.After pretreatment with sodium and carbamazepine,rats with seizures were used as drug-resistant epilepsy models.Rats with drug-resistant epilepsy were randomly divided into 5 groups and treated with carbamazepine,carbamazepine combined with specific inhibitor PDTC,and carbamazepine combined with low,medium and high dose Shufeng Zhijing prescription for 28 days.To observe the number of seizures and duration of seizures in rat models of drug-resistant epilepsy.After the gastrointestinal treatment was completed,6 rats in each group were randomly selected,and the cerebrospinal fluid of the rats was collected by microdialysis sampling technique,and the concentration of carbamazepine in the cerebrospinal fluid of the rats was detected by high performance liquid chromatography.Results:1.Efficacy of Shufeng Zhijing Recipe in the treatment of childhood epilepsy(1)Shufeng Zhijing Recipe has a certain curative effect on children with epilepsy,its obvious efficiency is 35.5%,and the total effective rate is 80.6%.The apparent efficiency of treating non-resistant epilepsy children is 45.9%,the total effective rate is 94.6%;the effective efficiency of treating drug-resistant epilepsy children is 20.0%,and the total effective rate is60.0%.(2)The results of syndrome analysis show that Shufeng Zhijing Recipe is more suitable for the syndrome of epilepsy with wind-heat syndrome,and the children have a history of fever convulsions in the past;the main cause of exacerbated fever is seizures;tonic-clonic As the main type of attack,the symptoms of traditional Chinese medicine are: loss of consciousness,binocular sight or strabismus,closed jaws,corners with or without salivation,rigidity and convulsions of limbs,often accompanied by external symptoms such as sneezing,stuffy nose,flow Runny nose,cough,red pharynx,pale or red tongue,white fur,floating or slippery pulse.2.Effect of Shufeng Zhijing Recipe on seizure behavior of drug-resistant epilepsy ratsExcept for normal control rats,rats in other groups have different degrees of seizures every week.(1)The impact of the number of attacks: the number of attacks in the medium and high dose groups of Shufeng Zhijing Fang in week 1 and week 2 is less than that in the drug-resistant model group and the positive control group(p<0.01);the low dose of Shufeng Zhijing Fang The number of attacks in the group was less than that in the positive control group(p<0.01),and there was no significant difference from the drug resistance model group(p>0.05).With the increase of the drug dose,the number of seizures of each dose group of Shufengzhijingfang showed a decreasing trend at week 3 and week 4(p<0.05);the number of seizures of Shufengzhijingfang medium and high dose groups was significantly less than that of drug resistance model group The positive control group(p<0.01)Shufengzhijingfang low-dose group had fewer attacks than the drug-resistant model group(p<0.01),and there was no significant difference from the positive control group(p>0.05).(2)Effect of seizure duration: The seizure duration of each dose group of Shufeng ZhijingFang gradually decreased with the increase of the drug dose at week 1 and week 2(p<0.01);the seizure duration of the positive control group was shorter than that of the other Group(p<0.05).With the increase of the drug dose,the duration of seizures gradually shortened in each dose group of Shufengzhijingfang in the third and fourth weeks,and there was no significant difference between the dose groups(p>0.05);the seizures continued in the high-dose group of Shufengzhijingfang The time was shorter than the drug resistance model group and the positive control group(p<0.01).3.Effect of Shufeng Zhijing Recipe on the concentration of carbamazepine in cerebrospinal fluid of drug-resistant epilepsy rats(1)The concentration of carbamazepine in the cerebrospinal fluid of the drug-resistant model group was lower than that of the other treatment groups,and it was statistically different from the positive control group and the high-dose group of Shufeng Zhijing Recipe(p<0.05);(2)The concentration of carbamazepine in the positive control group was higher than that of each dose group of Shufeng Zhijing Recipe,which was statistically different from the low and medium dose groups of Shufeng Zhijing Recipe(p<0.05),and the high dose group of Shufeng Zhijing Recipe No statistical difference(p>0.05);(3)With the increase of the dosage of Chinese medicine,the concentration of carbamazepine in the cerebrospinal fluid of each dose group of Shufengzhijingfang showed an increasing trend,but there was no statistical difference between the three groups(p>0.05).Conclusion:1.The effective rate of Shufeng Zhijing Recipe in treating children with epilepsy is35.5%,and the total effective rate is 80.6%.Shufeng Zhijing Recipe not only has a certain effect on the treatment of non-resistant epilepsy children,the total effective rate is 94.6%,but also has a certain effect on the treatment of drug-resistant epilepsy children,the total effective rate is 60.0%.2.In the clinic,Shufeng Zhijing Recipe is more suitable for epilepsy with wind-heat syndrome.Children have a history of febrile seizures;the main cause of exogenous fever is seizures;tonic-clonic seizures are the main type of seizures.3.Shufeng Zhijing Recipe can reduce the number of seizures and duration of seizures indrug-resistant epilepsy model rats;Shufeng Zhijing Decoction combined with carbamazepine can improve the cerebrospinal fluid of drug-resistant epilepsy rats The concentration of carbamazepine in China may be related to the mechanism of Shufeng Zhijing Recipe by regulating the NF-κB inflammatory signaling pathway in the brain,inhibiting related inflammatory cytokines,and down-regulating the level of P-gp,and then increasing the brain anti-epileptic drug kama The concentration of xiping is associated with reduced seizures. |
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