The Mechanism Of MiR-19a Regulate Osteoclast Differentiation In Goat Skull Defects

Objective To investigate the modulation mechanism of mi R-19 a in osteoclast differentiation.Methods After the establishing the goat models with skull defects,the skull tissues were collected from both goats with skull defects and healthy goats with normal development as samples.Gene sequencing analysis was then performed for the skull tissue samples,and the mi RNAs with obvious differential expressions were acquired.KEGG pathway analysis was carried out with the mi RNAs as the object,and the target genes of mi R-19 a were predicted by gene target prediction software,and the function of mi R-19 a in osteoclast differentiation and the its relationship with the target genes.Results mi R-19 a presented high expression in the skull tissues of the skull defect group;the mi RNAs with obvious differential expressions in the two samples presented enrichment in the osteoclast differentiation pathway;and the mi R-19a-modulated target genes play a role in the modulation pathway of osteoclast differentiation.Conclusion The highly-expressed mi R-19 a participates in the modulation of the target genes including TNF?,CYLD,PI3 K,AP1,NFATc1 and CTSK in the osteoclast differentiation pathway,and this modulation function may have influences on the metabolism of bone tissues.Objective To building a young sheep skull defect model,application of various biodegradable materials and titanium mesh for defect repair,collecting different repair 2 to 4 months after repair group animal serum samples,using enzyme-linked immunosorbent experimental detecting osteopontin in each group,insulin growth factor 1,osteocalcin concentration changes,to explore the influence of different biodegradable material for osteogenesis ability.Methods(1)choose 1 months baby sheep(small tail cold sheep)20,no male and female,randomly divided into five groups(composite materials,porous materials,high density materials,titanium net,blank group).(2)in the same position in each group made the same size of bone window,use different material to repair(blank group do not repair).(3)respectively in 2 and 4 months after repair to collect each baby sheep serum samples,enzyme-linked immunosorbent experiments,detection of osteopontin in each group,insulin growth factor 1,osteocalcin concentration changes.(4)dynamic change time-concentration curve drawing,to explore the influence of different biodegradable material for osteogenesis ability.Results(1)Compared with the blank control group,the levels of BGP,IGF-1 and OPN in the porous material group,low-hole material group and titanium mesh repair group were significantly higher than those in the blank control group(P <0.05).The expression of IGF-1 in the high-density group was statistically different(p <0.05).The expression of IGF-1 in the composite group was statistically different(p <0.05)).(2)The expression of BGP,IGF-1 and OPN in the high density group was statistically different from that in the blank control group(p < 0.05).The expression of IGF-1 in the composite group and the titanium mesh repair group was statistically different(p <0.05).(3)The results of the determination of serum in each group were compared with the blank control(P <0.05).The expression of OPN in the high-density group and the composite group was statistically different(p <0.05).The expression of OPN in the high-density group and the composite group was statistically different(p <0.05).Conclusion(1)porous materials,low-pore materials can stimulate the growth of skull tissue in a short time(2)titanium mesh on the growth of skull tissue can play a long stimulating effect,but its role gradually weakened(3)high-density materials And the composite material on the growth of skull tissue can play a long stimulating effect,and its role gradually increased.(4)Compared with other materials,high-density materials in the application of skull repair materials have the most broad prospects.