Correlation Between The Occurrence Of Infection And Lymphocyte Subsets In Patients With Liver Cirrhosis And The Characteristics Of Tcm Syndromes

Objective:To observe the relationship between the occurrence of infection and the level of lymphocyte subsets in patients with liver cirrhosis,and to study the correlation between the characteristics of TCM syndromes and the level of lymphocyte subsets in patients with liver cirrhosis,using SPSS21.0 software for data processing and synthesis Analysis,to explore the correlation between lymphocyte subsets and the occurrence of infection and TCM syndromes,aiming to analyze the immune factors of multiple infections in patients with liver cirrhosis,to provide reference for the early prevention and diagnosis and treatment of concurrent infections in patients with liver cirrhosis,At the same time,it will lay the foundation for the subsequent research on cirrhosis complicated with infection.Methods:The subjects in this trial were from patients with liver cirrhosis in the hepatobiliary ward of the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine between February 2018 and January 2020,excluding patients with malignant tumors,chronic kidney disease and other serious diseases Of patients.A total of 60 patients were divided into 25 cases in the infection group and 35 cases in the non-infection group.Lymphocyte subsets(flow cytometry),transaminase,transpeptidase,and albumin of two groups of patients were detected,and TCM consultation was conducted to record tongue veins and related syndromes for dialectics.Using computer statistical software SPSS21.0,through independent sample t test,x2 test and other statistical methods,analysis:(1)the relationship between transaminase,transpeptidase,serum albumin and TCM syndromes and infection in patients with liver cirrhosis,(2)concurrent infection and Differences of lymphocyte subsets in cirrhotic patients without concurrent infection.(3)Correlation between TCM syndrome types and lymphocyte subsets in patients with cirrhosis.Results:Part 1: Of the 60 cirrhotic patients,25 were in the infected group,15 were male(60.0%)and 10 were female(40.0%),with a maximum age of 74 years,a minimum age of 38 years,and an average age of 61.3 ±10.65 years old;35 cases in the non-infected group,23 males(65.7%)and 12 females(34.3%),with a maximum age of 74 years,a minimum age of 30 years,and an average age of 56.5±12.24 years.Statistical analysis showed that there was no statistical difference in gender and age between the two groups(P>0.05),which was comparable.The serological indexes of the two groups of patients were compared,and the statistical results showed that there was no statistical difference in the levels of ALT,AST,GGT,ALP,TBIL,and DBIL between the infected and non-infected groups(P>0.05).In the infection group,there were 5 cases of Child-Pugh A(20.0%),11 cases of Child-Pugh B(44.0%),9 cases of Child-Pugh C(36.0%),and the average Child-Pugh score It was 8.64±2.06 points.In the non-infected group,there were 11 cases of Child-Pugh A(31.4%),17 cases of Child-Pugh B(48.6%),7 cases of Child-Pugh C(20.0%),and the average Child-Pugh score The value is 7.77±2.06 points.The independent sample t test showed that the Child-Pugh scores of the two groups were not statistically different(P>0.05)and were comparable.There was a statistically significant difference in serum albumin(ALB)level between the infected group and the non-infected group(P<0.05),and the ALB level in the infected group(29.86±3.72g/L)was significantly lower than that in the non-infected group(32.32±4.33 g /L).There were statistical differences between the infected group and the non-infected group at the levels of CD3+/CD8+,CD3+/CD4+,CD3+CD4+/CD3+8+(P<0.05),and in CD3+,CD3-/CD19+,CD3-/CD16+56 + There is no statistical difference in the level(P>0.05).CD3+/CD8+(25.45±10.64%)in infected group was significantly higher than that in non-infected group(16.26±7.08%),CD3+/CD4+(40.17±7.98%)in infected group was significantly lower than that in non-infected group(47.78±11.44%),infected group CD3+CD4+/CD3+CD8+(1.91±0.96%)was significantly lower than the non-infected group(3.74±2.95%).In all 60 cases with liver cirrhosis,there are alcoholic cirrhosis group(33 cases),autoimmune cirrhosis group(16 cases),viral cirrhosis group(11 cases),and the comparison between the three groups is in CD3+,CD3+/CD4+ There was no statistical difference in CD3-/CD16+56+ levels(P>0.05),and there were statistical differences in CD3+/CD8+,CD3-/CD19+,CD3+CD4+/CD8+ levels(P<0.05).Part 2: In the 25 cases in the infection group,the order of the proportion of TCM syndromes is: Damp heat syndrome(52.00%)> Qi stagnation dampness syndrome(28.00%)> Deficiency of liver and kidney yin syndrome(16.00%)> Deficiency of spleen and kidney yang syndrome(4.00%).In the 35 cases in the non-infected group,the order of the proportion of TCM syndromes is: Qi stagnation dampness syndrome(45.71%)> Damp heat syndrome(25.71%)> Deficiency of liver and kidney yin syndrome(20.00%)> Deficiency of spleen and kidney yang syndrome Certificate(8.57%).In the comparison of the TCM syndrome types of the infected group and the non-infected group,there was no statistical difference between the Qi stagnation and dampness syndrome,Deficiency of liver and kidney yin syndrome,and Deficiency of spleen and kidney yang syndrome groups(P>0.05),and the infection group(52.00%)was damp and hot The proportion of syndromes was significantly higher than that of the non-infected group(25.71%),and there was a statistical difference(P<0.05).There were statistically significant differences in CD3+,CD3+/CD4+,CD3-/CD19+ levels among the four groups of patients(P<0.05),but there was no statistical difference in CD3+/CD8+,CD3-/CD16+56+,CD3+CD4+/CD3+CD8+ levels Difference(P>0.05).Conclusions: 1.When there is no statistical difference between the infected group and the non-infected group in age,gender,and Child-Pugh score,patients with cirrhosis with hypoproteinemia are more likely to develop infection,or patients with cirrhosis with infection are more likely to have low Proteinemia.2.In the case of no statistical difference in age,gender,Child-Pugh score between the infected group and the non-infected group,the percentage of CD8+ T cells increased,the number of CD4+ T cells decreased,and the ratio of CD4+T/CD8+T was biased Patients with low cirrhosis are more likely to develop infections.3.Patients with liver cirrhosis of different etiologies and syndromes have certain differences in the level of lymphocyte subgroups,and the etiology and syndrome types have a certain relationship with the immune status of patients.4.The proportion of damp-heat accumulation syndrome in the infected group was significantly higher than that of the non-infected group,suggesting that patients with liver cirrhosis with damp-heat accumulation syndrome are more likely to be infected.