| Objective:1.To screen differential endogenous metabolites in patients with acute brainsteminfarction,and to analyze the biomarkers obtained to explore the possible mechanism of acute brainstem infarction.2.Observe the changes of biomarkers in patients with acute brainstem infarctionbefore and after treatment in acupuncture group and control group,and explorethe possible intervention effects of acupuncture on acute brainstem infarction.Methods:1.Taking 27 clinical acute brainstem infarction patients and 25 normal healthypeople as the research objects,collecting 4-5 m L of fasting elbow venous bloodfrom the Participants in the morning,centrifuged by high-speed centrifuge,andtaking their serum as the test sample,using Ultrahigh performance liquidchromatography-quadrupole-time-of-flight mass spectrometry(UPLC/Q-TOF/MS)technology was used to analyze the metabolic profiles of clinical serum samplesfrom acute brainstem infarction group and healthy control group.The obtaineddata was exported using Mass Lynx(Version 4.1)software,and the multivariatestatistical analysis was performed on the exported data using SIMCA-P12.0statistical software(Swedish Umetrics),first by constructing an unsupervisedmodel Principal component analysis(PCA)Model,to investigate the overallmetabolic trends and contours of the overall sample,and then construct a partialleast squares-discriminant analysis(PLS-DA)model with a supervised model todistinguish between groups and find potential biomarkers Thing.Subsequently,anindependent sample t test was performed using SPSS17.0 software to determinewhether the metabolite had a statistically significant change,and the substancewith p<0.05 was selected as the metabolites with significant differences.Use them/z values of significantly different metabolites to search for possible substancesin the HMDB database and Chemspider database,and confirm candidatebiomarkers through information such as standards,MS/MS analysis,metabolitedatabase information,and literature.2.Taking 27 patients with acute brainstem infarction as experimental subjects,17patients in the acupuncture group were given acupuncture combined with basictherapy,and 10 patients in the control group were given basic therapy for 2 weeks.Serum was collected from patients with acute brainstem infarction before and aftertreatment as test samples,and metabolomics analysis was performed usingUPLC/Q-TOF-MS technology.The obtained data was exported using Mass Lynxsoftware,and the multivariate statistical analysis was performed on the exported datausing SIMCA-P12.0 statistical software to identify metabolites that contributedsignificantly to the difference in metabolic profiles before and after treatment inpatients with acute brainstem infarction.Based on the biomarkers of acute brainsteminfarction determined by "Experiment 1",find the biomarkers of acute brainsteminfarction according to the m/z value and retention time.Compared with the controlgroup,observe the biomarkers before and after treatment in the acupuncture groupThe trend of change and independent sample t test to determine whether themetabolite has a statistically significant change.Results: 1.Compared with the healthy control group,six differential metabolites werescreened out in the acute brainstem infarction group,mainly manifested asmethionine,tryptophan,phenylalanine,2-piperidinecarboxylic acid,phospholipidAcylethanolamine alkenyl,Lyso PC(18:1(11Z)/0:0)were significantly up-regulated.2.After basic treatment,patients with acute brainstem infarction had a significantcallback in 3 of the above 6 biomarkers,namely phenylalanine,methionine andLyso PC(18:1(11Z)/0:0)After the intervention of acupuncture,except for2-piperidinecarboxylic acid,there was no significant callback,the remaining 5 biomarkers were significantly corrected.Conclusion: 1.The selected biomarkers belong to glycerophospholipids and amino acids,indicating that acute brainstem infarction may be related to amino acidmetabolism and glycerophospholipid metabolism disorders.2.It is suggested that the number of metabolites regulated after acupunctureintervention is more,and the involved metabolic pathways are wider;regulatingthe metabolism of amino acids and glycerophospholipids,so that the body'sdisordered metabolism tends to be normal,may be important for the treatment of acute brainstem infarction with acupuncture Path of action. |
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