Effect Of Metformin On The Level Of F.prausnitzii In Intestinal Tract Of Db/db Mice And Its Related Mechanism

Objective:This study investigated the effects of metformin on SCFAs,intestinal mucosal barrier protection,and inflammatory factors in db/db mice based on the anti-inflammatory mechanism of F.prausnitzii.Methods:(1)Subjects:db/m+Na Cl group,db/db+Na Cl group,db/db+met group.FBG,TC,and TG levels were measured at 0,4,6 and 8 weeks after8 weeks of gavage.(2)Enzyme immunoassay was used to determine IL-10,IL-6,and insulin levels in serum of mice.(3)The levels of F.perusnitzii bacteria in fecal samples of mice at 0 to 8 weeks were determined by RT-PCR.(4)Colons and ileum sections were stained with HE staining and paraffin liver sections were stained with oil red O staining,and the pathological condition and fat content of the mice were examined.(5)The expression levels of claudin-1,claudin-2,zo-1,occlaudin and NF-?B in the colon tissues of mice were detected by western-blot.(6)The contents of acetic acid,propionic acid and butyric acid in 0-8-week mouse feces samples were determined by gas chromatography.Results:(1)Results of general biochemical indicators:compared with mice in the db/db+Na Cl group,fasting glucose level,triglyceride level,cholesterol level and body weight in the db/db+met group is significantly decreased,with statistically significant differences.(2)ELISA results:compared with mice in the db/db+Na Cl group,the serum level of pro-inflammatory factor IL-6 in the db/db+met group is decreased,with statistically significant difference.The level of anti-inflammatory factor IL-10 increases with statistically significant difference.Fasting insulin level is decreased,and the difference is statistically significant.(3)Rt-pcr results:Compared with mice in the db/db+Na Cl group,the level of F.prausnitzii bacteria in the feces of mice in the db/db+met group is significantly increased,with a statistically significant difference.(4)Pathological section results:compared with the mice in the db/db+Na Cl group,the colonic glands in the mice in the db/db+met group are arranged regularly,and the muscle layer is thickened.The structure of the ileum crypt is arranged in order and the muscular layer isthickened.Liver fat decreased significantly.(5)Western-blot results:compared with mice in the db/db+Na Cl group,claudin-1,zo-1 and occlaudin protein expressions in the colon tissues of db/db+met is increased,while claudin-2 protein is decreased,with statistically significant differences.The expression of inflammatory protein NF-?B is decreased,but the difference was not statistically significant.(8)GC test results: Compared with the db/db+Na Cl group,the acetic acid and butyric acid levels in the db/db + met group were significantly increased,and the difference was statistically significant.The propionic acid level was not significant Variety.Conclusion:The effects of metformin on anti-inflammatory,protecting the intestinal mucosal barrier and increasing the production of butyric acid are consistent with the functions of F.prausnitzii,suggesting that F.prausnitzii has played an important role in the improvement of glucose and lipid metabolism disorders in db/db mice.