Effect And Mechanism Of Schistosomia Polpeptide SJMHE1 On Airway Inflammation In Asthmatic Mice By Regulating The Expression Of MiRNA-17 And MiRNA-21

Objective Asthma is a common chronic respiratory disease,its essence is airway inflammation and the main pathophysiological characteristics are airway hyperreactivity and airway remodeling.The pathogenesis of asthma involves a variety of cells and cytokines,Studies have shown that multiple signaling pathways are involved in the occurrence and development of asthma.SJMHE1 is an immune regulatory peptide derived from Schistosoma japonicum HSP60(SJHSP60).The purpose of this study was to explore the intervention of SJMHE1 polypeptide on asthmatic mice,and to explore its mechanism at the cellular and molecular levels.In order to provide a new idea for the diagnosis and treatment of asthma.Methods 1.Eighty female BABL/C mice aged 6-8 weeks were randomly divided into four groups: control group,OVA group,OVA+PBS group,and OVA+SJMHE1 group.OVA was used to construct a asthma mouse model,and mice were subcutaneous injected with schistosomiasis polypeptide SJMHE1(OVA+SJMHE1 group)or PBS(OVA+PBS group)at the activation stage of OVA,their temperament and behavior changes were observed.2.HE staining of lung tissues was used to observe the pathological changes of lung tissues in each group and score the inflammation.3.The total number of bronchoalveolar lavage fluid cells in mice was counted and classified under the microscope.4.The Ig E levels of serum in each group were determined by enzyme-linked immunosorbent assay.5.Flow cytometry was used to detect the expression of Th1,Th2,Th17 and Treg in mouse spleen cells.6.The expression of IFN-??IL-4?IL-17?TGF-??Foxp3?GTAT3?ROR-?t?T-bet in mouse lung tissues was detected by real-time PCR.7.The expression of mi R-17 and mi R-21 in mouse lung tissues was detected by realtime PCR.8.Western blotting was used to detect the expression of p-STAT3 and PTEN protein in mouse lung tissues.Result 1.Compared with the Control group,the pathological manifestations of the lung tissues of the OVA group and the OVA +PBS group were significantly worse after HE staining,and the inflammatory score was increased.The inflammatory score of the OVA+SJMHE1 group was lower than that of the OVA group and the OVA+PBS group.2.Compared with the Control group,the total number of bronchoalveolar lavage fluid cells and the total number of eosinophils and percentage in the OVA group and the OVA+PBS group increased.Compared with the OVA+PBS group,the total number of cells,the total number of eosinophils and the percentage of bronchoalveolar lavage fluid cells in the OVA+SJMHE1 group decreased.3.Compared with the Control group,the levels of Ig E in the OVA group serum,the OVA+PBS group and the OVA+SJMHE1 group were increased,while there was no significant difference between the OVA,the OVA+PBS group and the OVA+SJMHE1 group.4.Compared with the Control group,the proportion of Th1 cells in the OVA and OVA+PBS group was decreased,the proportion of Th2 and Th17 cells increased,the proportion of Treg cells increased.Compared with the OVA group and the OVA+PBS group,the proportion of Th2 cells and Th17 cells decreased,and the proportion of Th1 and Treg cells increased.5.Compared with the Control group,the OVA and the OVA+PBS group lung tissue of mice showed that the m RNA expression levels of foxp3,TGF-?,T-bet,IFN-? were decreased,and GATA3,IL-17,IL-4,ROR-?t were increased;Compared with the OVA and the OVA+PBS group,the OVA+SJMHE1 group lung tissue of mice showed that the m RNA expression levels of foxp3,TGF-?,T-bet,IFN-? were increased,and GATA3,IL-17,IL-4,ROR-?t were decreased.6.Compared with the Control group,the expression levels of mi R-17 and mi R-21 in the lung tissues of the OVA group and the OVA+PBS group were increased.Compared with the OVA group and the OVA+PBS group the expression levels of mi R-17 and mi R-21 in the lung tissues of the OVA+SJMHE1 group were all decreased.7.Compared with the Control group,the expression level of p-STAT3 protein in the lung tissues of the OVA group and the OVA+PBS group was up-regulated and the expression level of PTEN protein was down-regulated,while the expression level of pSTAT3 protein in the lung tissues of the OVA+SJMHE1 group was down-regulated and the expression level of PTEN protein was up-regulated.Conclusion The asthma mice model was successfully constructed by OVA in the experiment.SJMHE1 peptide can effectively intervene OVA-mediated asthma in mice.SJMHE1 can regulate,mi R-21,mi R-17 in m RNA and PTEN,p-STAT3 protein expression by reversing Th1/Th2 and Th17/Treg cell imbalance in asthmatic mice,and inhibit the airway inflammation in asthmatic mice.