Long Noncoding RNA 10913 Regulates Lipopolysaccharide-induced Inflammatory Response In Pulmonary Macrophages

Objective Lnc RNA is a long non-coding RNA,which is widely involved in the regulation of various biological processes in the body.Studies have shown that lnc RNA is an important regulator of inflammatory response.To establish the cell model of sepsis acute lung injury.To screen differentially expressed lnc RNAs by high-throughput sequencing.To investigate the regulation of lnc RNA10913 on lipopolysaccharideinduced inflammatory response in pulmonary macrophages.Methods LPS-stimulated cells were used to construct an inflammatory response model of alveolar macrophages,and lnc RNA high-throughput sequencing technology was used to detect the changes of lnc RNA expression profile in cells before and after LPS stimulation.Build lnc RNA10913 and interference of expression plasmid vector,then transfection to alveolar macrophages.The experiment was divided into 6 groups: 1.Control group;2.LPS treatment group;3.Overexpression vector +LPS treatment group;4.Interference vector +LPS treatment group;5.oe-lnc RNA10913 +LPS treatment group;6.sh-lnc RNA10913 transfection +LPS treatment group.The interference effect of sh-lnc RNA10913 plasmid was detected by RT-PCR,and the over-expression and interference efficiency were verified by Q-PCR.The protein expressions of inflammatory factors TNF-? and IL-6 in the supernatant of cells were detected by ELISA.Results 1.Lnc RNA expression profile was significantly changed after stimulation of alveolar macrophages.Compared with the blank control group,717 lnc RNAs were differentially expressed after LPS stimulation of alveolar macrophages 9h,in which 446 lnc RNAs were up-regulated by more than 2 times and 271 lnc RNAs were down-regulated by more than 2 times,indicating that lnc RNA expression changes were related to the inflammatory response of alveolar macrophages.2.The expression of lnc RNA10913 increased by 1.27 and 1.92 times as compared with the control group after LPS stimulation of alveolar macrophages for 2h and 9h,indicating that lnc RNA10913 is closely related to the inflammatory response of alveolar macrophages.3.The expression of lnc RNA10913 in the oe-lnc RNA10913 group was significantly increased compared with that in the overexpression vector group after transfection with the oe-lnc RNA10913.In addition,the expression of TNF-? and IL-6 proteins in the supernatant after LPS induction was also significantly up-regulated,indicating that overexpression of lnc RNA10913 could enhance the LPS-induced inflammatory response of alveolar macrophages.4.The expression of lnc RNA10913 in the sh-lnc RNA10913 group was significantly decreased compared with that in the Interference vector group after transfection with sh-lnc RNA10913.In addition,the expression of TNF-? and IL-6 proteins in the supernatant after LPS induction was also significantly decreased,indicating that the silencing of lnc RNA10913 could inhibit the LPS-induced inflammatory response of alveolar macrophages.Conclusion Lnc RNA10913 was highly expressed in LPS-induced macrophages.Overexpression of lnc RNA10913 can enhance the inflammatory response of macrophages induced by LPS.Silencing lnc RNA1091310913 inhibited LPS-induced macrophage inflammation.The discovery of lnc RNA10913 provides a possible direction for the treatment of sepsis ALI/ARDS.