Preparation And Properties Of Hyaluronic Bioadhesive

Tissue adhesive is a type of biomedical material that can be directly applied to the human body.By using tissue adhesive,the wound can be closed more quickly,and it can also speed up the repair of wounds in later treatment.At the same time,when dealing with some acute visceral injuries,tissue adhesive can prevent blood loss in a timely manner.Thanks to this,tissue adhesive is considered to be one of the most promising research fields.However,the existing tissue adhesives generally have problems such as poor wet adhesion properties,incomplete fit with tissues,and insufficient sealing performance,which limit further applications of tissue adhesives.Therefore,it is of great significance to develop tissue adhesives with good adhesion properties and biocompatibility.Aiming at the problems of poor wet adhesion performance,insufficient biocompatibility,and low adhesion strength of tissue adhesives,this paper proposes to use hyaluronic acid,an extracellular matrix component widely presented in the human body,as the basis for dopamine-grafted aldehyde-based hyaluronic acid,maleated hyaluronic acid and dopamine-grafted maleated hyaluronic acid were prepared by combining with acylation,and a series of hyaluronic acid tissue adhesive.The rheology,scanning electron microscope,universal stretching machine and other instruments were used to systematically study the gel dynamics,micro-morphology,onboard shear strength,and burst pressure of the hyaluronic acid binder system.The progress made in this article is as follows:?1?Dopamine-grafted aldehyde-based hyaluronic acid tissue adhesive:Two dopamine-grafted aldehyde-based hyaluronic acids?DAHA?with a degree of substitution of 45%and25%,respectively,were synthesized.When the alkaline sodium periodate solution is mixed,it can quickly form a gel within 40 s.A large number of dopamine groups provide excellent wet adhesion strength?90 kPa?and high adhesion energy(384.6±26.0 J·m^-2),Higher burst pressure?65 kPa?.In addition,in terms of biological performance,the hydrogel can promote the growth and attachment of L929 cells well,and does not show cytotoxicity.?2?Maleic hyaluronic acid tissue adhesive:chemical modification using the abundant hydroxyl sites on the hyaluronic acid skeleton,successfully grafting carbon-carbon double bonds on the hyaluronic acid skeleton,and synthesizing two carbon-carbons the degree of double bond substitution was 2.15 and 1.1 maleated hyaluronic acid?MHA?,respectively.Under the ultraviolet light?Wavelength:320-400 nm,light intensity:30 mW/cm²?,the light crosslinks to form a gel.At the same time,it exhibits excellent adhesion strength?21.4±2.2kPa?,high adhesion energy(150.2±8.3 J·m^-2),and short molding time?280 s?.In terms of biological properties,MHA hydrogel can promote the growth and attachment of human umbilical vein endothelial cells well,and it does not show toxicity.?3?Dopamine grafted maleyl hyaluronic acid tissue adhesive:After successfully synthesizing MHA,MHA with a degree of substitution of 2.15 was selected for further chemical modification to graft the dopamine group.After two steps of reaction,the simultaneous syntheses were successfully synthesized.Dopamine with carbon-carbon double bonds and dopamine groups is grafted with maleic acylated hyaluronic acid?MHA-DP?.Under the rultraviolet light?Wavelength:320-400 nm,light intensity:30 mW/cm²?and oxidizing environment,the gel can be formed in situ,and the adhesive ability can be maintained in the wet state.It also has high adhesion strength?36.2±3.1 kPa?,high adhesion energy(367.3±15.2 J·m^-2),short molding time?290 s?and suitable burst pressure?31.13 kPa?.To sum up:the three hydrogel systems based on DAHA,MHA,and MHA-DP all show good tissue adhesion properties and biocompatibility,and can maintain continuous adhesion ability in wet conditions.The proportion of the components can meet the needs of different tissues for adhesion ability,and has broad application potential in the field of biomedical adhesives.The three adhesive systems each have different advantages,in terms of adhesion strength and underwater adhesion strength:DAHA>MHA-DP>MHA;in terms of adhesion strength:DAHA>MHA-DP>MHA;in terms of burst pressure:DAHA>MHA-DP>MHA;in gel elasticity Aspect:MHA-DP>MHA>DAHA.When used in different locations and environments,the three adhesives developed in this article can be used together to meet different needs.