| Obesity is one of the most important issues in global society.Excessive energy intake,weight gain,and subsequent obesity are positively related to disease,disability,and mortality.Recent studies have shown that obesity is closely related to intestinal flora.Imbalance of intestinal flora and reduction of host intestinal barrier function can cause abnormal lipid metabolism,which in turn triggers obesity and causes metabolic damage.Diet is the main factor affecting the intestinal flora.Irrational diets can cause intestinal flora imbalances.Several studies have shown that remodeling the intestinal flora through intermittent fasting can significantly improve metabolic syndromes such as obesity and is of great significance to human health.Intermittent fasting programs reduce weight by reducing energy intake while maintaining adequate nutrient intake.The weight loss caused by this energy-limiting regimen is not only associated with improved clinical biochemical indicators,but also linked to many health benefits,including not only improved glucose metabolism and insulin sensitivity,reduced oxidative stress and inflammatory effects,but also improved cardiovascular disease and blood lipids,extending life.This article describes the role of intestinal flora in the treatment of obesity by intermittent fasting,and proposes that in the study of obesity,the combination of intestinal flora and intermittent fasting can be used to promote clinical transformation.In this study,methods such as qPCR,Western Blot,transcriptome sequencing,immunohistochemistry,and microbial sequencing were used to explore the role and mechanisms of intestinal microbes in intermittent fasting-mediated metabolic benefits.This study provides a theoretical basis for reducing the incidence of obesity through the combination of intestinal microorganisms and intermittent fasting in the future.The results of this study are as follows:1.Intermittent fasting inhibits intestinal lipid absorption by regulating intestinal flora.Mice fed with high fat were treated with unrestricted feeding and intermittent fasting to detect the inhibitory effect of intermittent fasting on obesity.After IF,the symptoms of obesity were reduced,the fasting blood glucose was improved,and the weight of adipose tissue was reduced.The intestinal tight junction protein m RNA and protein levels were significantly increased(P <0.05),and intestinal HE staining showed an extremely significant increase in small intestinal villi height(P<0.01).In addition,after intermittent fasting treatment,the expression of de novo lipid synthesis-related genes DGAT and FASN decreased significantly(P<0.05),the expression of intestinal fatty acid absorption protein FATP and CD36 were significantly reduced,and chylomicrons and lipoproteins transferred lipoproteins APOE expression increased significantly,APOB and APOA expression decreased significantly(P <0.05).Oil red O staining of the small intestine also indicates that IF can reduce intestinal lipid accumulation caused by high-fat feeding.In addition,antibiotic treatment and colonization experiments with Akkermansia confirmed that intestinal microbes play a role in IF-mediated weight loss.2.Intermittent fasting promotes browning of white fat by affecting intestinal microorganisms.Under the condition of high-fat feeding,unrestricted feeding and intermittent fasting treatment were respectively performed to detect the inhibitory effect of intermittent fasting on obesity.After IF,the m RNA and protein levels of subcutaneous fat browning genes in mice were significantly increased(P<0.05).HE staining showed that adipocytes changed from large to small.In addition,antibiotic treatments and custom experiments with Akkermansia have confirmed that gut microbes play a role in IF-mediated weight loss.This experiment demonstrates that intermittent fasting can reduce intestinal lipid deposition and promote browning of white fat by influencing intestinal microorganisms,providing research ideas for the treatment of metabolic diseases such as obesity. |
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