Expression Of HArd1 Genes In Human Esophageal Squamous-cell Carcinoma Cells And Its Effects On Cell Proliferation,Migration And Invasiveness.

Objective :To study the expression of n-α-acetyltransferase 10 protein(h Ard1)in human Esophageal Squamous-Cell Carcinoma(ESCC)cells and its influence on the invasion and proliferation of ESCC,so as to provide some experimental and theoretical basis for the further study of ESCC precise treatment.Methods :1.ESCC cell culture,q RT-PCR method to detect the expression level of h Ard1,2.The constructed plasmids were transfected to ESCC cells to make the expression level of h Ard1 in ESCC cells different,MTT method to detect the cell proliferation,scratch test and Transwell method to detect the cell invasiveness of each group.3.Spss20.0 was used to analyze the expression of h Ard1 and its correlation with the proliferation,invasion and metastasis of ESCC cells.Results :1.The expression of h Ard1 in ESCC cells was high.2.When the expression level of h Ard1 increased,the proliferation of ESCC cells decreased,while when the expression level of h Ard1 decreased,the proliferation of ESCC cells increased.3,when the expression level of h Ard1 increased,the invasiveness and metastasis ability of ESCC cells decreased,while when the expression level of h Ard1 decreased,the invasiveness and metastasis ability of ESCC cells increased.Conclusion :The high expression of h Ard1 in ESCC cells.h Ard1 is negatively correlated with the proliferation of ESCC cells,and negatively correlated with the invasiveness and metastasis of ESCC cells.