Experimental Study On The Expression Of Bnip3 And Bcl-2 In Alcoholic Cardiomyopathy

Objective: Aim at the alcoholic cardiomyopathy of rats,establish the related model,and study the expression mechanism of BNIP3 and Bcl-2 in the myocardium of alcoholic cardiomyopathy rats.Methods: Sixteen adult male SD rats of clean grade(weight 200-250g)were fed adaptively for one week(full price pellet feed,free drinking water).One week later,8rats were randomly selected as model group,and the remaining 8 rats as normal control group.The rats in the model group were given intragastric administration of quantitative liquor at 9:00 and 16:00 every day(liquor dose 20g/(kg.d)= ethanol dose 10g/)(kg.d));normal control group was given the same amount of distilled water at the same time.The general condition of rats was recorded and observed.After 15 days,the rat model of ACM was established successfully through the detection of serological indexes such as myocardial enzymes,echocardiography and pathological examination.The expression of Bnip-3 and Bcl-2 in rat myocardium was detected by immunohistochemistry and Western Blot method,and their relationship was analyzed to explore the possible mechanism of cardiomyocyte apoptosis in alcoholic cardiomyopathy from the protein level.Results: 1.The general condition of rats: all the rats in the normal control group survived and their body weight increased significantly,while in the model group,3 rats died,and their body weight increased slowly,even some showed negative growth.There was a significant difference in BW(body weight)(P < 0.05)between the two groups,but there was no significant difference in HW(heart weight)and HW/BW(P>0.05);2.Echocardiography: compared with the normal control group,the cardiac morphological indexes LVDD and LVSD in the model group were increased(P <0.05),IVST,LVPWTs and LVPWTd were decreased,and the functional indexes LVFS and LVEF were decreased(P < 0.05);3.Serum myocardial enzymes(AST,LDH,HBDH,CK): compared with the control group,the serum myocardial enzymes in the model group increased significantly(P < 0.05);4.Pathology: there was no abnormality in myocardial HE staining in the normal control group.Myocardial HE staining in the model group showed obvious cardiomyocyte hypertrophy,nuclear pyknosis,part of muscle fiber rupture and inflammatory cell infiltration;5.Immunohistochemicaldetection: compared with the normal control group,the expression of Bnip3 in the model group was enhanced,the staining deepened,and coarse granules could be seen;the expression of Bcl-2 was weakened,the staining was light and uneven distribution;6.Western Blot detection: compared with the normal control group,the expression of Bnip3 was increased(P<0.01)and the expression of Bcl-2 was decreased(P<0.01)in the model group,and there was a negative correlation between the expression of Bnip3 and Bcl-2(P<0.01).Conclusion: 1.The rat model of alcoholic cardiomyopathy was established successfully through the detection of serological indexes such as myocardial enzymes,echocardiography and pathological examination;2.Cardiomyocyte apoptosis induced by alcohol is related to the abnormal expression of apoptosis-related genes Bnip3 and Bcl-2: the expression of Bnip3 is enhanced in alcoholic cardiomyopathy,which promotes cardiomyocyte apoptosis;the expression of Bcl-2 in alcoholic cardiomyopathy decreases and inhibits cardiomyocyte apoptosis;3.There was a negative correlation between the expression of Bnip3 and Bcl-2 in alcoholic cardiomyopathy.