Expression And Mechanism Of LncRNA In Osteosarcoma

Objective:It has been found that long non-coding RNA(LncRNA)SNHG5 and Gclnc1 play an important role in tumors.However,the role and mechanism of lncRNA SNHG5 and Gclnc1 in osteosarcoma is not clear.The purpose of this study is to explore the expression and regulation mechanism of SNHG5 and Gclcn1 in osteosarcoma.Methods:The bioinformatics analysis of osteosarcoma chip based on online database GEO was used to predict the expression and prognosis of lncRNA SNHG5 in osteosarcoma.20 osteosarcoma tissue samples from the third affiliated Hospital of Nanchang University and Jiangxi traditional Chinese Medicine Hospital were collected,and the expression of lncRNA Gclnc1 in osteosarcoma samples was detected by realtime fluorescence quantitative PCR(RT-PCR).143 B,MG63(gene knockout)and U2 OS,U2R(overexpression)cell lines were selected to study the function of SNHG5.MG63(gene knockout)and U2OS(overexpression)cell lines were selected to study the function of Gclnc1.After knockdown and overexpression of SNHG5,the effects of SNHG5,miR-212-3p and SGK3 on the growth of osteosarcoma cells were investigated by MTT method,clone formation test,flow cytometry,Transwell test and cell scratch test.The interaction between lncRNA SNHG5 and miR-212-3p was detected by RT-PCR,Western blotting(Western blot)analysis and luciferase report test.After knockdown and overexpression of Gclnc1,the cell viability was detected by MTT method.Colony formation assay was used to detect cell proliferation.Apoptosis was detected by flow cytometry and cell migration and invasion were detected by Transwell assay.RIP assay was used to detect the expression of Gclnc1 and NONO combined with,Western blot to detect the expression of NONO.Animal experiments in vivo confirmed that Gclnc1 inhibited the growth of osteosarcoma in vivo.Results:In this study,knockout of lncRNA SNHG5 and Gclcn1 inhibited the growth and metastasis of osteosarcoma cells,while overexpression produced the opposite result.In mechanism,lncRNA SNHG5 acts as a sponge to adsorb miR-212-3p and inhibit miR-212-3p/SGK3 signal pathway.The overexpression and inhibition of miR-212-3p can reverse the tumor-promoting and inhibitory effects of SNHG5,respectively.The function of SNHG5 can be saved by miR-212-3p and regulate the growth and metastasis of osteosarcoma cells through SGK3,the downstream target of miR-212-3p.On the other hand,we detected the collected osteosarcoma specimens by RT-PCR experiment and found that Gclnc1 was significantly expressed in osteosarcoma tissues compared with adjacent tissues.In mechanism,lncRNA Gclnc1 can bind to RNA binding protein NONO to regulate the progression of osteosarcoma.In addition,our results showed that in the nude mouse experiment,compared with the control group,the volume and weight of tumor in the Gclnc1 knockdown group were significantly lower than those in the control group.Conclusion:To sum up,this study confirmed that lncRNA SNHG5 may regulate the proliferation and metastasis of OS cells through the miR-212-3p/SGK3 axis.The expression of lncRNA Gclnc1 in osteosarcoma is significantly increased,and the high expression of lncRNA Gclnc1 promotes the proliferation and metastasis of osteosarcoma cells by regulating NONO.These findings may provide new targets for future clinical treatment.