Study On The Antibacterial Activities Of The Novel Antibacterial Agent Imidazolium Salts Against Common Bacteria In Postoperative Spinal Infections

Objective:To evaluate the antibacterial effects of the novel antibacterial agent imidazolium salts(IBN-1,PIM-45)on common bacteria in postoperative spinal infections.Methods:(1)The minimum inhibitory concentrations(MICs)of imidazolium salts(IBN-1,PIM-45),vancomycin,ampicillin,ciprofloxacin,polymyxin B,and cefotaxime against common bacteria in postoperative spinal infections were measured.(2)The effects of imidazolium salts(IBN-1 PIM-45),vancomycin,ampicillin,ciprofloxacin on the formation of methicillinresistant Staphylococcus aureus(MRSA)(ATCC43300)mature biofilms were determined by crystal violet staining.(3)MTT experiments,crystal violet staining,and scanning electron microscopy(SEM)were used to determine the damage of imidazolium salts(IBN-1,PIM-45),vancomycin,ampicillin and ciprofloxacin on MRSA(ATCC43300)mature biofilms.Results:(1)IBN-1 and PIM-45 demonstrated broad-spectrum antibacterial activities,which presented strong antibacterial effects on both grampositive and gram-negative bacteria in the experiment.And they demonstrated strong antibacterial effects on both standard strains and clinically isolated drug-resistant strains in the experiment.The antibacterial effects of IBN-1 and PIM-45 on staphylococcus aureus in the experiment were comparable to vancomycin,and the antibacterial effects of IBN-1 and PIM-45 on some strains in the experiment were stronger than that of ampicillin,ciprofloxacin,polymyxin B and cefotaxime.(2)IBN-1,PIM-45,and vancomycin demonstrated strong inhibitory effects on the formation of the mature biofilms of MRSA(ATCC43300),and their inhibitory effects were comparable.The higher the drug concentration,the stronger the inhibitory effect on the formation of the mature biofilms of MRSA(ATCC43300),while ampicillin and ciprofloxacin had no inhibitory effects on the formation of the mature biofilms of MRSA(ATCC43300).(3)IBN-1,PIM-45,and vancomycin demonstrated strong destructive effects on the mature biofilms of MRSA(ATCC43300),and their destructive effects were comparable.The higher the concentration of the drug,the stronger the destructive effect on the mature biofilms of MRSA(ATCC43300).Ampicillin and ciprofloxacin presented destructive effects on MRSA(ATCC43300)mature biofilms when the concentration reached 2000 ?g/mL.Conclusions:(1)The novel antibacterial agent imidazolium salts(IBN-1,PIM-45)presented strong antibacterial effects on common bacteria in postoperative spinal infections,and they demonstrated strong antibacterial effects on both standard strains and clinically isolated drug-resistant strains in the experiment.The antibacterial effects of IBN-1 and PIM-45 on staphylococcus aureus in the experiment were comparable to vancomycin,and the antibacterial effects of IBN-1 and PIM-45 on some strains in the experiment were stronger than ampicillin,ciprofloxacin,polymyxin B and cefotaxime.(2)IBN-1 and PIM-45 demonstrated strong inhibitory effects on the formation of MRSA(ATCC43300)mature biofilms,and they also demonstrated strong destructive effects on the mature biofilms of MRSA(ATCC43300).The effects of IBN-1 and PIM-45 on the formation and destruction of mature biofilms of MRSA(ATCC43300)were comparable to vancomycin and stronger than ampicillin and ciprofloxacin.In summary,IBN-1 and PIM-45 may become potential drugs for the prevention and treatment of postoperative spinal infections.