Expression And Significance Of Chk1 And Chk2 In Children With Acute Lymphoblastic Leukemia

Objective:To investigate the expression level of checkpoint kinase 1?Chk1?and checkpoint kinase 2?Chk2?in children with acute lymphocytic leukemia?ALL?,to analyze the correlation between Chk1 and Chk2 in children ALL and clinical characteristics,early treatment response and clinical risk stratification,and to explore the significance of them in the pathogenesis of ALL in children.Methods:Eighty-three children with ALL diagnosed in hematology-oncology department of Shenzhen Children's hospital from November 2017 to November 2019 were selected,including 68 B-ALL children and 15 T-ALL children,and twelve immune thrombocytopenia?ITP?,a non-malignant disease of the blood system,were selected as the control group.The bone marrow samples from children in the case group and the control group were collected,and were used to isolate white blood cells.The relative expression levels of Chk1 and Chk2 mRNA in bone marrow samples of the new diagnosed case group and the control group were detected by real-time fluorescence quantitative polymerase chain reaction?RT-qPCR?.The relative expression levels of Chk1 and Chk2 in ALL children were analyzed and described by the method of 2^-?35??35?Ct,and the correlation between their relative expression levels and clinical indicators were researched.Results:1.The expression level of Chk1 in ALL children was[1.677?0.688,2.502?],significantly higher than that in the control group[0.273?0.159,0.512?],and the difference was statistically significant?P<0.05?,but there was no statistical difference in the expression level of Chk1 between b-all and t-all children?P>0.05?.2.There were statistically significant differences in the expression level of Chk1 in different clinical risk stratification levels?P<0.05?,with the highest expression level in the high-risk group[2.441?1.608,5.502?],followed by the medium-risk group[1.953?0.749,2.447?]and the lowest expression level in the low-risk group[0.975?0.205,1.866?].3.The expression level of Chk1 was correlated with the age of ALL children and the proportion of bone marrow promyelocytic lymphocytes at first diagnosis?r values were0.243 and 0.269,P values were<0.05?.4.The expression level of Chk2 in ALL children was[1.395?0.746,2.536?],higher than that in the control group[0.228?0.185,1.416?],and the difference was statistically significant?P<0.05?,but there was no statistical difference in the expression level of Chk2in b-all and t-all children?P>0.05?.5.Chk2 was correlated with clinical risk stratification,with the highest expression level in the high-risk group[2.727?1.259,3.698?],followed by the medium-risk group[1.403?0.744,2.019?],and the lowest expression level in the low-risk group[0.833?0.715,1.948?].However,there were only statistical differences between the high-risk group and the low-risk group,and the high-risk group and the medium-risk group?P<0.05?.6.The change trend of Chk1 and Chk2 expression levels in ALL children was consistent and positively correlated?r=0.467,P<0.001?.Conclusion:1.Chk1 and Chk2 are highly expressed in ALL bone marrow of children.2.The expression levels of Chk1 and Chk2 are related to the clinical risk stratification in children with ALL,which may be a marker of poor prognosis.3.Dysregulation of Chk1 and Chk2 expression may play a role in the occurrence and development of ALL in children.