Porous Scaffolds From Droplet Microfluidics For Prevention Of Intrauterine Adhesions

Objective: Severe intrauterine adhesions have a great negative impact on women's psychological and reproductive health.It is still a significant challenge to prevent postoperative intrauterine adhesions due to the deficiencies of various clinical preventive measures.So,we propose a method for preparing a novel drug-loaded three-dimensional porous scaffold based on a microfluidic droplet template,to explore the preliminary effect of the scaffold in the prevention of intrauterine adhesions.Methods: Combined with the microfluidic technology of emulsion and the characteristics of composite biomaterials GelMA and sodium alginate,three-dimensional tissue engineering scaffolds were prepared.By optimizing the ratio between various materials,the scaffolds were imparted with good compressibility,flexibility,mechanical properties and porous structure.Owing to the suitable external-internal connected open structures,the scaffold could be controllably loaded drug and release sustainably.Secondly,by changing the collection device of the microfluidic droplets,porous scaffolds in line with the shape of uterine cavity could be further achieved,which is conducive to blocking the wound surface more effectively,and draining the uterine cavity ooze simultaneously.Finally,in order to validate the specific function of the porous scaffold in prevention of intrauterine adhesion,the system was tested in SD female rat models.Results: 1.Preparation of three-dimensional porous tissue engineering scaffolds with pore sizes ranging from 80?m?350?m;2.The ratio of hydrogel was optimized.When the ultimate optimized concentration configuration of 10%wt GelMA,2%wt Na-alginate,2%wt SDS,2%wt F108,1%wt HMPP and 83%wt deionize water were used,the scaffold had better performance;3.Test the performance of the scaffold,showing that the scaffold has good properity of compressibility,swelling,degradability,and biocompatibility;4.The loading efficiency and release behavior of the porous scaffolds were tested,the loading efficiencycould be up to nearly 30%,and the cumulative release amount could reach up to around 40percent;5.The results of rat experiment showed that the system had the ability to prevent intrauterine adhesions,improve neovascularization,cellularization of damaged tissue,and promote the repair of damaged endometrium.Conclusions: The drug-loaded three-dimensional porous scaffold has good plasticity and biocompatibility.To a certain extent,it can effectively prevent intrauterine adhesion and promote the repair of damaged endometrium.