The Novel Electrovhrmical Method For Tumor Marker Detection Based On Multifunctional Nanomaterials

Human epididymis 4（HE4）is a novel ovarian cancer marker which is not expressed in normal tissues but highly expressed in tumor tissues,especially in ovarian serous carcinoma.Compared with the currently recognized ovarian cancer marker glycan antigen-125（CA125）,HE4 is much more specific for ovarian cancer diagnostic.Although HE4 has a relatively high content in the serum of ovarian cancer patients,as a tumor marker,it still needs high sensitive detection technology which can accurately and quantitatively detect its low concentration to realize the early diagnosis of ovarian cancer.At present,the commonly used HE4detection techniques are mainly automatic chemiluminescence method and enzyme-linked immunosorbent method.These methods require expensive instruments and reagents,and the detection process is complex and time-consuming.In recent years,electrochemical immunosensors have been widely developed and studied for their advantages of simplicity,low cost,high sensitivity and easy miniaturization.With the rapid development of nanotechnology,Alloy nanomaterials with various morphologies have showed their potential application in electrochemical signal amplification.Here,the proposed electrochemical immunosensor for HE4 detection was based on the trimetallic AgPtCo nanodendrites（NDs）and magnetic nanocomposites（Fe₃O₄@SiO₂@Au,MNCs）.The trimetallic AgPtCo NDs were synthesized as powerful redox probe and highly efficient signal amplifier for electrochemical signal readout.The established method,drawing upon the strong electrochemical activity of AgPtCo NDs and the enrichment and separation effect of MNCs,showed a high sensitivity,excellent selectivity,repeatability and stability.The detection range of this method was 0.001–50 ng mL^-1 and the detection limit was as low as 0.487pg mL^-1.Besides,the developed sensor was applied for the HE4 accurate quantitative detection in the serum of clinical ovarian cancer patients,and exhibited great anti-interference ability.The good performance of this method was attributed the large surface area and stable and efficient electrochemical activity of AgPtCo NDs,as well as the enrichment and separation of trace substance in complex matrices of MNCs.Thus,this AgPtCo NDs and MNCs based electrochemical immunosensor might become a powerful alternative approach for ultrasensitive detection of various serum biomarkers associated with early diagnosis and treatment of diseases,which is of great clinical significance.