Study On The Relationship Between TREM2 Gene Polymorphism And Alzheimer's Disease In Qinghai Area

Objective To investigate the association between polymorphisms at rs75932628(R47H),rs142232675(D87N),rs201258663(T66M),rs143332484(R62H),and rs104894002(Q33X)in the triggering receptor expressed on myeloid cells 2(TREM2)gene and Alzheimer's disease(AD)in Qinghai Han population.To clarify the relationship between AD and the above loci of TREM2 gene in Qinghai Han nationality.Methods From June 2017 to December 2019,44 AD patients were collected from the Affiliated Hospital of Qinghai University and other hospitals in Qinghai Province as the case group,and another 56 healthy subjects were collected from the Physical Examination Center of the Affiliated Hospital of Qinghai University as the control group,which was matched with the age and gender of the case group.Peripheral blood DNA was extracted,and the target gene was amplified by PCR and the above five loci of rs75932628(R47H),rs142232675(D87N),rs201258663(T66M),rs143332484(R62H),and rs104894002(Q33X)in the TREM2 gene were genetically sequenced by sanger method to compare the relationship between different genotypes and alleles and the risk of Alzheimer's disease.Statistical analysis was performed using IBM SPSS 22.0 statistical software.Results 1.There were no significant differences in gender,age,smoking history,drinking history,and education level between the two groups(P>0.05);there were significant differences in MMSE scores between the two groups(P<0.001).2.comparison of TC,TG,HDL,LDL,and CRP between the two groups revealed no significant differences in TC(P= 0.139),TG(P = 0.171),HDL(P= 0.366),LDL(P= 0.306),and CRP(P= 0.120)between the two groups(P> 0.05).3.Rs75932628(R47H)showed GG genotype in all subjects of this study,and no GT or TT genotype was found;rs142232675(D87N)and rs143332484(R62H)showed GG genotype in all subjects of this study,and no GT or TT genotype was found;rs201258663(T66M)and rs104894002(Q33X)showed CC genotype in all subjects of this study,and no CA or AA genotype was found.In summary,the rs75932628(R47H),rs42232675(D87N),rs201258663(T66M),rs143332484(R62H),and rs104894002(Q33X)polymorphisms of the TREM2 gene were not found to be associated with AD in the Qinghai Han population in this study.Conclusion In this study,the rs75932628(R47H),rs142232675(D87N),rs 201258663(T66M),rs143332484(R62H),and rs104894002(Q33X)polymorphisms of the TREM2 gene were determined,and TREM2 gene mutations were found in all study subjects of the Qinghai Han population,and TREN2 gene mutations may not be a risk gene for AD in the Qinghai Han population.