Research Of Expression Level Of High Mobility Group Protein B1 In Peripheral Blood Of Patients And The Correlation With Helper T Cell 17/Regulatory T Cell With Primary Sj?gren's Syndrome

Objective:To investigate the relationship between the high mobility group box protein B1 level in peripheral blood and the balance of helper T cell 17 /regulatory T cells in patients with primary Sj?gren's syndrome,and the correlation with the related cytokines in order to study the role of HMGB1 in the pathogenesis of pSS.Methods:From May 2018 to May 2019,40 patients with primary Sj?gren's syndrome who were newly diagnosed in the Department of Rheumatology and Immunology in the Second Hospital of Shanxi Medical University were selected as the pSS group.The physical examination was performed at the same time in the physical examination center of our hospital.Twenty patients served as a control group.Elbow venous blood was collected from the subjects,Th17 and Treg cells were detected by flow cytometry,and Th17/Treg ratio was calculated.Serum interleukin-17(IL-17),IL-6,IL-10 concentration was detected by cytometric bead array(CBA).Enzyme-linked immunosorbent assay for detection of HMGB1,transforming growth factor-b(TGF-b)concentration.Each of the above indicators was compared between groups,and the correlation between HMGB1 and the above-mentioned cells and related cytokines was analyzed.Results:The levels of HMGB1,Th17 and Th17/Treg in the pSS group were higher than those in the control group,while the Treg level was lower than that in the control group.The levels of IL-17,IL-6 and TGF-b in the pSS group were higher than those in the control group,while the levels of IL-10 were lower than those in the control group.Correlation analysis showed that HMGB1 was positively correlated with Th17,Th17/Treg and its related cytokines IL-17 and IL-6(r = 0.738,0.502,0.762,0.578,P<0.05).HMGB1 was negatively correlated with Treg and IL-10 levels(r =-0.657,-0.686,P <0.05),and had no significant correlation with TGF-b.Conclusion:The HMGB1 of patients with primary Sj?gren's syndrome is abnormally high in peripheral serum,and there is obvious T cell immune abnormality compared with healthy people,suggesting that HMGB1 may participate in the development of primary Sj?gren's syndrome by causing an imbalance of Th17/Treg.