Study On Neurons In Visual Cortex Of Glaucoma Mice Using Electrophysiological Recording Technology

Purpose: Glaucoma is one of the most blinding eye diseases in the world.It can cause irreversible visual field defects and visual impairment.Current research has confirmed that glaucoma can cause damage to retinal ganglion cells,optic nipples,and optic nerves,and that damage can be extended to higher visual centers such as the lateral geniculate body and the visual cortex.At present,the pathological changes of glaucoma in the entire visual pathway have not been fully elucidated.The purpose of this project is to creatively study the electrophysiological and histomorphological characteristics of neurons in the primary visual cortex(V1)of DBA / 2J glaucoma model mice.Methods: In this experiment,DBA / 2J glaucoma model mice were used as the experimental group(7 mice),and C57 BL / 6J mice were used as the normal control group(8 mice).Extracellular microelectrode recording method was used to collect the electrophysiological response of V1 neurons in living mice.We recorded five properties of V1 neurons,including optimal orientation,optimal temporal frequency,optimal spatial frequency,classical receptive field and response changes under different contrast stimuli.Finally,the brain sections of the two groups of mice were observed morphologically by immunofluorescence staining and Nissl staining.Results: A total of 149 neurons were collected from V1 of DBA / 2J mice and C57 BL / 6J mice(including 81 neurons of DBA / 2J mice and 68 neurons of C57 BL / 6J mice).compared to C57 BL / 6J mice,V1 neurons of DBA/2J mice had: Weaker orientation selectivity?lower optimal spatial frequency?larger classical receptive field size ? spontaneous discharge level of neuron decreased and Positive expression for nuclear protein of neurons reduced,and number of neurons was smaller,with neuron's structural abnormal and intracellular Nissl bodies decreased.Conclusions: The electrophysiological activity of neurons in V1 region of DBA /2J glaucoma model mice shows abnormal characteristics in many aspects.At the same time,positive expression for nuclear protein of neurons reduced,and number of neurons was smaller,with neuron's structural abnormal and intracellular Nissl bodies decreased.It indicates that,during the course of glaucoma disease,the functional characteristics and histomorphological damage of neurons in the V1 region may happen at the sametime.This provides a basis for us to a better understanding of pathogenesis of glaucoma and the pathological changes of its higher visual centers at the cellular level.