Glucose Responsive Photonic Nanochains For Real-time Monitoring In Seconds

Glucose in the blood?blood glucose?is the main energy-supplying substance of the human body.If the body's blood sugar secretion disorder?diabetes?causes a variety of complications and is life-threatening.However,current technology still cannot cure diabetes,and diabetes can only be controlled by real-time monitoring of blood sugar levels.In the past few decades,a variety of glucose detection technologies and sensors have been developed.Among them,the most widely used in clinical is still the electrochemical glucose sensor,but it faces many difficult problems,such as signal interference,signal drift,tissue loss and so on.Subsequently,the development of optical glucose detection technology has provided a new research direction for researchers.Among them,the glucose responsive photonic crystal sensor combines a photonic crystal with a glucose responsive gel,and changes the gel volume caused by the change of the glucose concentration,so that the lattice constant of the photonic crystal changes,and then the color change of the sensor.However,most of the traditional glucose-responsive photonic crystals exist in the macroscopic form of gel films or microspheres of tens to hundreds of micrometers?thickness or diameter?,which have the disadvantages of long glucose response time and complicated preparation,and still cannot meet the real-time monitoring of glucose.Therefore,real-time monitoring technology for glucose development is imminent.Our laboratory has used hydrogen bond template polymerization to prepare photonic nanochains that respond to pH,temperature,and solvent.Since their gel shell thickness is only a few ten nanometers,the response time to the stimulus source has been increased to Second level.If a glucose-responsive gel is coated as a shell around the chain structure to form a glucose-responsive photonic nanochains,it is expected that the response rate will be increased to the second order.However,hydrogen bond template polymerization is not suitable for the preparation of glucose responsive photonic nanochains.In response to this problem,this paper innovatively developed a universal DMSO solvation layer method and successfully prepared a new class of second-order glucose responsive photonic crystal nanochain sensors.The main research contents and results of this thesis are as follows:?1?In water and DMSO solution,the chain formed by Fe₃O₄@PVP particles under magnetic field is used as a template,AAPBA is enriched around the chain due to DMSO and AAPBA factors,and Fe₃O₄@PVP@PAAPBA photonic nanochains is obtained by in-situ polymerization.The effect of the ratio of water and DMSO on the structure of photonic chain was studied,and chain reaction mechanism analysis shows that the key to the preparation of such photonic nanochains is the choice of DMSO solvation layer and pro-DMSO hydrophobic monomer.Subsequently,the glucose response performance of the photonic chain was studied,which showed that the response time had reached the second level in the test environment pH 10.0.?2?The HEAA/AM hydrophilic monomer was introduced on the basis of?1?,and Fe₃O₄@poly?AAPBA-co-HEAA?and Fe₃O₄@poly?AAPBA-co-AM?photonic nanochains were prepared by DMSO solvation layer method.Making Fe3O4@PVP@PAAPBA photonic nanochains has a lower pKa value?the environment is reduced from pH 10.0 to 8.0?,and the glucose response range is improved???0-50 is increased from 33 nm to 176 nm and 147 nm,respectively?.Taking the Fe₃O₄@poly?AAPBA-co-HEAA?photonic chain as an example,the preparation and glucose response mechanism were elucidated,and the changes of glucose response performance with various influencing factors were studied.The results show that the photonic nanochains realizes the change of color from bright green,bright yellow,orange yellow,orange red to red when the glucose concentration is 0-15 mM,which realizes the second-order detection of naked eye.Fe₃O₄@poly?AAPBA-co-HEAA?photonic nanochains are expected to be applied to second-level glucose real-time monitoring due to their simple preparation,short cycle,high efficiency,fast response to glucose,sensitive,visible,full spectrum,small size and friendly test environment.