MEA-based Neurobiosensor System And Its Application In Brain Slices

The research of neuron information transmission mechanism is related to human understanding of the brain,brain science and the development of human society and human health.It is of great significance for the treatment of cranial nerve diseases.In this paper,we mainly focus on the acquisition of neuro-electrical signals,and creatively propose a bio-brain sensor system based on Microelectrode Array(MEA),which is applied to the acquisition and research of nerve electrical signals in isolated brain slices to study brain nerves.Meta information transfer mechanism.The overall design of the sensor system,the whole system is divided into two large parts,the neural electrical signal acquisition and stimulation two parts,with electrical signal acquisition as the main design core,divided into micro-electrode acquisition unit,electrical signal conditioning unit,multi-channel control Units and dedicated data processing software units are the four main system units.According to the overall design block diagram of the sensor system,each system unit is realized.Firstly,the implementation of the microelectrode acquisition unit,the type of the microelectrode array is selected,and a special interface board is designed.Secondly,the design,simulation and implementation of the signal conditioning unit are performed.These include three levels of operational amplification,active low-pass filtering and power frequency notching.These three modules are the core of signal acquisition;then the design and implementation of multi-channel acquisition units.The realization of multi-channel acquisition is based on data.The acquisition card is used to control a pair of 32:1 multiplex selectors.This article also creatively adds timedivision multiplexing technology,successfully using 32 analog channels to acquire 32 signals;the last dedicated data software uses MFC.Realization,using MFC programming fast and convenient and strong compatibility features,efficiently achieve a series of functions such as data acquisition,real-time display and storage.In the bio-validation experiment,the preparation and culture of rat brain slices were carried out.In order to maintain the physiological activity of brain slices,a perfusion and ventilation system and a microscope-based real-time observation system were specially designed,which finally formed a complete set.Biosensor system experimental platform.In order to prove the physiological activity of brain slices,the activity of brain cells in each group was identified by staining experiments on brain slices of group cultures.The results showed that this experiment can maintain physiological activity and preparation of brain slices and culture.Physiological experiments.The nerve electrical signals on the brain slices were collected and analyzed for the discharge pattern.The brain slices were electrically stimulated,and the neuron discharge response of the brain slices during the stimulation process was collected,and compared with the stimulation signals,and the regularity was analyzed.The experimental results show that the sensor system designed in this paper has feasibility and applicability,and can be used for data acquisition and analysis of brain nerve electrical signals.