Study On The Relationship Between Chronic Intermittent Hypoxia Oxidative Stress And Fasting Blood Glucose And Fasting Plasma Insulin Level In SD Rats

ObjectiveA rat model of chronic intermittent hypoxia(CIH)was established to provide a reliable experimental platform for study of the pathophysiological development mechanism of obstructive sleep apneahypopnea syndrome(OSAHS).The blood of the control group and chronic intermittent hypoxia group were collected and the levels of fasting blood glucose(FPG),fasting insulin(FINS),total antioxidant capacity(T-AOC),reactive oxygen species(ROS)and malondialdehyde(MDA)in different periods were determined to explore the possible pathophysiological mechanisms between OSAHS,oxidative stress and diabetes.This study could further elucidate the role of oxidative stress in the mechanism of chronic intermittent hypoxia on blood glucose regulation and provide a new basis for possible intervention node.MethodsForty 8-week-old healthy male Sprague-Dawley(SD)rats weighing 190-210 g were randomly divided into unhandled control group(unhandled control,UC)and chronic intermittent hypoxia group(chronic intermittent hypoxia,CIH)after one week of adaptive feeding.UC group was given normal feeding,and CIH group was intermittent intermittent hypoxia for 8 hours.The rat heart blood of two group were collected and the levels of fasting blood glucose(FPG),fasting insulin(FINS),total antioxidant capacity(T-AOC),reactive oxygen species(ROS)and malondialdehyde(MDA),respectively,The blood of two group were collected on the 0th,10 th,20th,30 th day respectively to detect the levels of fasting blood glucose(FPG),fasting insulin(FINS),total antioxidant capacity(T-AOC),reactive oxygen species(ROS),malondialdehyde(MDA).Result(1)MDA increased at 10 d,20d and 30 d in CIH group compared with the UC group(P<0.05).(2)The fluorescence of ROS increased at 10 d,20d and 30 d in CIH group compared with the UC group(P<0.05).(3)T-AOC decreased at 10 d,20d and 30 d in CIH group compared with the UC group(P<0.001).(4)The concentration of FINS in the CIH group at 10 d,20d and 30 d were higher than that of the UC group(P<0.05).(5)The concentration of FPG in the CIH group at 10 d,20d and 30 d were higher than that of the UC group(P<0.05).(6)MDA concentration was higher than 0d(P<0.001)in intermittent hypoxia for 10 d,20d and 30 d,and the intermittent anoxic 30 d was significantly higher than the 10 d MDA concentration(P<0.001).(7)The ROS fluorescence value increased at 0d,20 d and 30 d after intermittent hypoxia(P<0.001),and the intermittent hypoxia 30 d was significantly higher than the 10 d ROS fluorescence value(P<0.001).(8)The level of T-AOC showed a downward trend at 0d,10 d,20d and 30 d in CIH group,and the different of any two groups were statistically significant(P<0.05).(9)The concentration of FINS increased at 30 days compared with 0d in CIH group(P<0.05).(10)The concentration of FPG increased at 10 d,20d and 30 d compared with 0d in CIH group(P<0.05).(11)There was in the level of MDA,ROS,T-AOC,FINS and FPG have no significant difference among 0d,10 d,20d and 30 d in the UC group(P>0.05).(12)With the increase of MDA concentration and ROS fluorescence,the concentration of FPG and FINS increased.With the increase of FPG and FINS concentration,the MDA concentration and ROS fluorescence increased in the CIH group(P<0.05).(13)With the decrease of T-AOC,the concentration of FPG and FINS increased.With the increase of the concentration of FPG and FINS,T-AOC decreased(P<0.05).ConclutionChronic intermittent hypoxia can cause oxidative stress indicators changes in SD rats,such as increased malodialdehyde,reactive oxygen species,hyperinsulinemia and elevated blood glucose,reduced total antioxidant capacity.With the increase of hypoxia time,fasting blood glucose,fasting insulin,reactive oxygen species and malondialdehyde showed an upward trend,and the total antioxidant capacity showed a decreasing trend.Fasting blood glucose and fasting insulin were positively correlated with reactive oxygen species and malondialdehyde,and negatively correlated with total antioxidant capacity.Chronic intermittent hypoxia can induce changes in fasting insulin and fasting blood glucose levels in rats through oxidative stress.