PdIrBP Mesoporous Nanospheres Combined With Superconductive Carbon Black For The Electrochemical Determination And Collection Of Circulating Tumor Cells

An integrated electrochemical immunoassay is described for the determination of circulating tumor cells（CTCs）.For the first time,Ketjen black（KB）,which is a superconductive carbon,was incorporated with Au nanoparticles（AuNPs）and used to modify the surface of gold electrodes.A cocktail of anti-epithelial cell adhesion molecules（EpCAM）and anti-vimentin antibodies was chosen to capture the CTCs.Palladium-iridium-boron-phosphorus alloy-modified mesoporous nanospheres（PdIrBPMNS）served as a catalytic tag to amplify the current signal.Glycine-HCl（Gly-HCl）was used as an antibody eluent to release and collect the captured CTCs from the electrodes for further clinical research without compromising cell viability.The response of the method increased linearly from 10 to 1×10⁶ cells mL^-1 CTCs,while the detection limit was calculated to be as low as 2 cells mL^-1.This method was successfully used to determine CTCs in spiked blood samples and demonstrated good recovery.