Effects Of Luteolin On Proliferation And Apoptosis Of Human Ovarian Cancer Cellline SKOV3

ObjectiveTo observe the effects of Luteolin on the proliferation and apoptosis of human ovarian cancer SKOV3 cells,and to explore differentially expressed genes?DEGs?closely related to the effects of luteolin through transcriptome sequencing.To explore the molecular mechanism of luteolin in regulating ovarian cancer proliferation and apoptosis,to provide new ideas for the treatment of ovarian cancer,and to provide scientific theoretical basis for the development and application of luteolin.Methods1.Human ovarian cancer SKOV3 cells were cultured in vitro.The cells were divided into blank control group and luteolin groups(10,20,40?mol·L^-1),and drug was given for 48h.CCK-8 assay was used to detect cell proliferation inhibition rate;EdU was used to detect cell proliferation ability;Flow cytometry?FCM?was used to detect cell cycle and apoptosis rate;The morphological changes of cells were observed by Hochest33342staining.2.DEGs of the blank control group and the 40?mol·L^-1 luteolin group intervention for 48h in SKOV3 cells were analysised by transcriptome sequencing.According to the functional annotations obtained by the DEGs in the gene ontology database?GO?and the Kyoto Encyclopedia of Genes and Genomes?KEGG?,the genes related to luteolin regulating cell proliferation and apoptosis were excavated.According to the transcriptome sequencing results,the expressions of CDKN1A,CDC20,GADD45A,ATF4,DDIT3,HSPA1A,HSPA2,HSPA8,HSPB1 mRNA in each group of cells were detected by RT-qPCR.Results1.Compared with the control group,the cell inhibition rate of luteolin groups was significantly increased?P<0.05?,which was in a concentration-dependent manner;The rate of EdU-positive cells of luteolin groups was significantly decreased?P<0.05?;The percentage of G₀/G₁phase cells of luteolin groups was significantly decreased?P<0.05?,and the percentage of cells in S phase was significantly increased?P<0.05?,showing a concentration-dependent manner;Hochest33342 staining showed apoptosis morphology in luteolin groups;Apoptosis rate of luteolin groups was significantly increased?P<0.05?,showing a concentration-dependent manner.2.1476 DEGs were obtained by transcriptome sequencing analysis,including CDKN1A,CDC20,GADD45A,ATF4,DDIT3,HSPA1A,HSPA2,HSPA8,HSPB1 that regulate cell proliferation and apoptosis.Compared with the control group,the expression levels of CDKN1A?GADD45A?ATF4?DDIT3 mRNA were increased?P<0.05?,the expression levels of CDC20?HSPA1A?HSPA2?HSPA8?HSPB1 mRNA were decreased?P<0.05?.ConclusionLuteolin can inhibit the proliferation and promote apoptosis of SKOV3cells,and its mechanism may involve the multi-gene regulation of CDKN1A,CDC20,GADD45A,ATF4,DDIT3,HSPA1A,HSPA2,HSPA8,and HSPB1.