Study On The Mechanism Of Autophagy In Human Metapneumovirus Infection

Human metapneumovirus(hMPV)has been discovered for 19 years since 2001.The virus was first isolated by Dutch scholars from the airway secretions of patients with respiratory diseases,and subsequently showed an epidemic trend worldwide.Infants,young children and those patients with impaired immune function were easily susceptible.Although hMPV has been discovered for many years,most of the research is still focused on epidemiological aspects,we still know little about the mechanism of its intracellular infection,and there are no appropriate vaccines and efficacious drugs to prevent or control the replication of virus in body after hMPV infection.In previous studies,our research group found that the virus can enter the cells by endocytosis and is closely related to lipid rafts.However,there is no understanding of the relevant mechanisms after entry into the cell.In this study,the intracellular mechanism induced by hMPV infection was studied.GFP-RFP-LC3 fluorescence plasmid transfection,autophagy related gene detection and pathway protein detection,it was found that hMPV infection could cause intracellular autophagy and this process was related to JNK,MEK/ERK pathway.Induced autophagy in turn could inhibit virus replication.It is also found that simvastatin can induce autophagy through AKT/mTOR pathway to reduce virus replication and has certain anti-inflammatory effect,which provides an experimental basis for the drug research of human partial lung virus and broadens the range of simvastatin.