Meta-analysis Of The Concentration Of SCFAs In The Feces Of Female Anorexia Nervosa Patients

Objective:The concentrations of short chain fatty acids(SCFAs),the main bacterial metabolites in the feces of women with anorexia nervosa(AN),were systematically evaluated to provide evidence of evidence-based medicine for clinical treatment.Methods:PubMed,The Cochane Library,Embase,cnki,wanfang data knowledge service platform,chongqing weipu,6 databases were searched.Search terms included anorexia nervosa and short chain fatty acids.Similar search words are connected by"OR",while different search words are connected by"AND".The search time is from the self-built database to February 1,2020.There was no language restrictions.Retrieve all case-control trials(CCT)and randomized-controlled trials(RCT)reported in feces of anorexia nervosa patients of short chain fatty acid of bacteria metabolites,through the formulation into and exclusion criteria,select standard research,extract the data and the use of RevMan5.3software for data statistics analysis,through the I~2 statistic test evaluated the heterogeneity of among the studies,use the random effects model has obvious heterogeneity,using a fixed effect model when has no obvious heterogeneity,and using STATA 12.1 software perform the Egger's test to access the publication bias of studies.Meanwhile,the changes of intestinal flora diversity and abundance in female anorexia nervosa patients were qualitatively analyzed.Results:1.A total of 3 case-control trials were included in the study analysis.The results of the meta-analysis using the random effect model showed that:compared with the healthy control group,the total concentration of SCFAs(SMD=-0.69,95%CI:[-1.35,-0.03],P=0.04<0.05,I~2=74%),concentration of acetate(SMD=-0.87,95%CI:[-1.69,-0.06],P=0.03<0.05,I~2=82%),concentration of propionate(SMD=-0.67,95%CI:[-1.23,-0.11],P=0.02<0.05,I~2=65%),concentration of butyrate(SMD=-0.65,95%CI:[-1.24,-0.06],P=0.03<0.05,I~2=69%)all decrease in the feces of female AN patients,with statistically significant differences,while the concentration both of isobutyrate(SMD=0.90,95%CI:[-0.05,1.86],P=0.06>0.05,I~2=87%)and iso-valerate(SMD=0.24,95%CI:[-0.32,0.79],P=0.41>0.05,I~2=67),and the difference was not statistically significant.2.Qualitative analysis of the changes of bacteria count and species in the feces of female patients with AN showed that the count of firmicutes represented by Ruminococcus,Roseburia,Clostridium decreased significantly,the total bacterial count also decreased,and the number of M.smithii methanobacteria in archaea increased significantly.The overall diversity of bacteria was decreasing.Conclusion:The concentration of SCFAs in the feces of female AN patients is lower than that of normal people,and the intestinal flora is disordered.SCFAs may serve as a specific diagnostic indicator of AN and provide a theoretical basis for the treatment of probiotics and other biological preparations in female AN patients.However,the number of included studies in this paper is small,and it still needs to be confirmed by the inclusion of more high-quality large sample studies in the future.