Expression Of Basic Fibroblast Growth Factor (bFGF) In Hippocampus Of Hyperbilirubinemia Newborn Rats And Its Significance

Objective:To observe the relationship of basic fibroblast growth factor(bFGF)expression with apoptosis of hippocampus in hyperbilirubinemia neonatal rats and hyperbilirubinemia brain injury,and to explore the role and mechanism of bFGF.Methods:A total of 120 neonatal rats aged 7 days old were selected as experimental subjects and randomly assigned.The number of each group was equal to 40,which were respectively: control group(group T0,rats were intraperitoneally injected with equal volume of normal saline),model group(group T1,rats were intraperitoneally injected with 100μg/ g bilirubin solution),and model group(group T2,rats were intraperitoneally injected with 150μg/ g bilirubin solution).According to the experimental requirements(8rats were selected and to execute from 6h,12 h,24 h,48 h and 72 h after the modeling),each group was divided into 5 subgroups.HE staining was performed on the brain tissue of each group.To observe pathological changes in the hippocampus under light microscope.The number of apoptosis of brain cells in the hippocampus was determined by TUNEL method.The number of bFGF expression positive cells in the hippocampus was measured by immunohistochemistry,and to observe the relationship between apoptosis and bFGF expression.Results:Compared with group T0,group T1 and group T2 at the same time point,both the apoptosis number of brain cells and the expression of bFGF increased(P < 0.05);and they were significantly higher in group T2 than in group T1(P < 0.05);There was a positive correlation between theapoptotic rate and bFGF expression in the hippocampus(r =0.994,P < 0.01).Conclusion:1.With the increase of the amount of bilirubin entering brain tissue,the number of hippocampal cell apoptosis increased,indicating that there was cell apoptosis during the injury process.2.With the increase of the amount of bilirubin entering the brain,the expression of bFGF in the hippocampus also increases,which indicates that bFGF is closely related to cell apoptosis,and this factor may participate in its pathophysiological process and play an important neuroprotective role.