Changes Of ROS And GSH In The Process Of Liver Fibrosis

Objective:Liver fibrosis is a dynamic pathological state caused by various acute and chronic liver injuries.It is resulted in the activation of HSCs caused by inflammation and the excessive accumulation of ECM.At present,oxidative stress is the common pathogenesis of many chronic liver diseases,and oxidative stress itself is the imbalance between ROS production and antioxidant defense system.Therefore,we studied the changes of ROS and GSH levels in serum during the process of liver fibrosis in rats and their relationship,as well as the relationship between normal people and Chronic hepatitis B cirrhosis.To explore the significance of ROS and GSH in the formation of liver fibrosis,To provide a new scheme for early clinical treatment of liver fibrosis Methods:1.Animal experiment: 34 healthy and clean male rats were raised,.They were randomly divided into two groups: 6 rats in the control group and 28 rats in the model group.The rats in the model group were injected subcutaneously with 40% CCl4 oil,and the first injection was 5ml / kg,then the amount was reduced to 2ml / kg,twice a week.The rats in the control group were injected subcutaneously with the same dosage of oil.Six rats were killed in the model group at the 2nd,4th,6th and 8th week after CCl4 injection respectively.Six rats were killed in the control group at the 8th week.The serum and liver tissue at the same location were taken.The liver tissues of each group were stained with HE and Masson The concentrations of ROS and GSH were measured by ELISA and colorimetry respectively.2.Clinical experiment: 20 serum samples of normal control group and 20 serum samples of hepatitis B cirrhosis group were collected,and the concentrations of ROS andGSH were measured by enzyme-linked immunosorbent assay and colorimetry respectively.Results:Animal experiment:(1)Pathological manifestations of liver tissue: the normal control group showed normal liver tissue structure and orderly arrangement of liver cells;the model group showed distorted structure of liver lobules at 8 weeks,forming complete and incomplete interlobular space between the portal vein and the central septum of the portal vein,infiltration of inflammatory cells around the blood filled vessels,thus forming a regenerative nodule and forming a false lobule,The mold was made successfully.(2)With the establishment of liver fibrosis model,the ROS level of model group was 1.84 ± 0.69,1.72 ± 0.65,2.48 ± 1.14,1.82 ± 0.46,respectively high concentration compared with the control group(1.56 + 0.84),the discrepancy was statistically sense(P = 0.000,0.000,0.010,0.000,respectively,which were less than 0.05);GSH level was 18.39 ±6.86,17.17 ± 6.50,24.80 ± 11.44,18.22 ± 4.58,respectively There was no meaning discrepancy between the two groups(P = 0.525,0.712,0.38,0.54,all greater than 0.05).(3)The results of clinical collection of specimens showed that the blood serum ROS concentration in cirrhotic patients was evidently higher than that in normal healthy controls(55.01 + 40.09),the discrepancy was statistically sense(P < 0.05);the serum GSH concentration in cirrhotic patients(0.577 + 0.233)was higher than that in healthy controls(0.425 + 0.107),the discrepancy was not statistically meaning(P > 0.05).(4)Pearson correlation analysis: there was a positive correlation between ROS and GSH in the process of liver fibrosis in rats,the difference was statistically significant(r = 0.81,P < 0.05).Conclusion:1.The levels of GSH and ROS in the model group were higher than those in the normal group,and GSH was positively correlated with ROS,which might be involved in the development of liver fibrosis.2.The concentrations of ROS and GSH in patients with chronic cirrhosis were higher than those in healthy controls.