Expression And Clinical Significance Of S100A8 And S100A9 In Helicobacter Pylori Associated Gastritis

Objective: To explore the expression pattern and clinical significance of S100A8 and S100A9 in H.pylori associated gastritis,so as to provide theoretical basis for the pathogenesis,diagnosis and treatment of H.pylori associated CAG.Methods: The expression of S100A8 and S100A9 in the gastric mucosa tissue of 101 patients with chronic gastritis were determined by immunohistochemistry(in mean optical density,MOD),and the expression of S100A8 and S100A9 at m RNA level in the gastric mucosa tissues of 48 patients were detected by RT-PCR.And the results combined with pathological diagnosis of routine staining and clinical H.pylori infection data for analysis.Manner Whitney U test,Kruskal Wallis H test and Spearman rank correlation were performed for statistical analysis.Results: 1.Among 101 patients,59 cases were chronic atrophic gastritis(CAG group)and 42 cases were chronic non-atrophic gastritis(NAG group);59 cases were H.pylori positive(H.pylori positive group)and 42 cases were H.pylori negative(H.pylori negative group).There were statistically significant differences in the mean optical density(MOD)of S100A8 and S100A9 between CAG group and NAG group(0.10,0.07 to 0.13 vs.0.09,0.06 to 0.10 and 0.13,0.08 to 0.15 vs.0.09,0.07 to 0.10,respectively),and between H.pylori positive group and H.pylori negative group(0.11,0.10 to 0.13 vs.0.07,0.06 to 0.08 and 0.13,0.10 to 0.15 vs.0.07,0.07 to 0.08,respectively),(U=754.00,602.00,5.00 and 40.00,all P<0.01).2.There were statistically significant differences in the mean optical density(MOD)of S100A8 and S100A9 between H.pylori positive patients(34 cases)and H.pylori negative patients(25 cases)in CAG group(0.13,0.11 to 0.14 vs.0.07,0.07 to 0.08 and 0.15,0.14 to 0.16 vs.0.08,0.08 to 0.09,respectively),and between H.pylori positive patients(25 cases)and H.pylori negative patients(17 cases)in NAG group(0.10,0.09 to 0.10 vs.0.06,0.05 to 0.07 and 0.10,0.10 to 0.11 vs.0.067,0.065 to 0.068,respectively)(U=1.00,0.00,0.00 and 0.00,all P<0.01).The results of Kruskal Wallis H test indicated that the differences among four groups were statistically significant(H=84.78 and 89.64,both P<0.01).3.There were statistically significant differences in the expression of S100A8 and S100A9 at m RNA level between CAG group(24 cases)and NAG group(24 cases)(0.12,0.06 to 1.31 vs.0.05,0.03 to 0.08;0.19,0.03 to 0.43 vs.0.03,0.01 to 0.09),and between H.pylori positive group(24 cases)and H.pylori negative group(24 cases)(0.45,0.10 to 1.90 vs.0.05,0.03 to 0.08;0.36,0.24 to 0.81 vs.0.03,0.01 to 0.04),(U=55.00 and 74.00,P<0.05 and 0.01 and U=19.00 and 2.00,both P<0.01).4.There were statistically significant differences in the expression of S100A8 and S100A9 at m RNA level between H.pylori positive patients(12 cases)and H.pylori negative patients(12 cases)in CAG group(0.85,0.27 to 2.28 vs.0.06,0.03 to 0.09 and 0.39,0.25 to 0.87 vs.0.03,0.02 to 0.05),and between H.pylori positive patients(12 cases)and H.pylori negative patients(12 cases)in NAG group(0.09,0.05 to 0.28 vs.0.04,0.03 to 0.07 and 0.20,0.09 to 0.65 vs.0.01,0.01 to 0.03),(U=5.00,2.00,0.00 and 0.00,all P<0.01).The results of Kruskal Wallis H test showed that the differences among four groups were statistically significant(H=20.43 and 24.15,both P<0.01).5.The expression of S100A8 and S100A9 were significant positive correlated at both protein level and m RNA level(r=0.899 and 0.903 respectively,both P<0.001).Conclusion: S100A8 and S100A9 may involve in the inflammation process of H.pylori-infected gastric mucosa and inducing the occurrence of gastric epithelial proliferation disorder.The high expression level of S100A8 and S100A9 may be one of mechanisms of intrinsic glands reduction and CAG genesis.S100A8 and S100A9 are promising diagnostic and follow up bio-markers and potential therapeutic targets for CAG.