| Objective:In this study,the optimal modeling method of contrast-induced acute kidney injury was explored by comparing the degree of creatinine elevation and pathological changes in the different models of contrast-induced acute kidney injury.Methods:40 healthy adult SD rats were randomly divided into 5 groups: group A blank control group: normal feeding;group B simple contrast group: normal feeding + diatrizoate;group C classic model group: normal feeding + indomethacin + NNitro-L-arginine methyl ester base + diatrizoate;group D dehydration model group: water-free + furosemide + diatrizoate;group E classic + dehydration model group: water-free + furosemide + indomethacin + N-nitro-L-arginine methyl ester + diatrizoate.In the 5 groups of SD rats,2 ml of orbital venous blood was taken on the 1st,6th,and 11 th day.On the 11 th day,the bilateral kidneys of the rats were taken out after anesthesia.Blood samples were assayed for creatinine and urea nitrogen to assess changes in rat kidney function.Superoxide dismutase and glutathione levels were measured in the left kidney tissue to assess inflammation and oxidative stress.The pathological changes of renal tubules were observed by HE staining.The apoptosis was observed by TUNEL staining.The expression of apoptosis-related proteins such as caspase-3 and caspase-9 in renal tissues was determined by immunohistochemistry.Results:(1)Modeling success rate: After Fisher exact probability method was used to calculate the difference between each group(P<0.05).Group A modeling success rate is 0%,group B modeling success rate is 12.5%,group C modeling success rate is 25%,group D modeling success rate is 75%,and group E modeling success rate is 100%.(2)The degree of SCr and BUN elevation: The SCr increased by 153.10% from baseline after 48 hours of injection of contrast agent in group E,and the Scr increased by 103.47% from baseline after 7 days of injection of contrast agent.There were statistical differences between group E and groups A,B,C,D in the increase of 48 h SCr(1.52 ± 0.29 vs 0.16 ± 0.24;1.52 ± 0.29 vs 0.32 ± 0.16;1.52 ± 0.29 vs 0.44 ± 0.94;1.52 ± 0.29 vs 0.89 ± 0.59;p < 0.05);The BUN at 48 h after injection of contrast agent in group E was 342.09% higher than the baseline,and the BUN at 7 days after injection of contrast agent was 77.34% higher than the baseline.The increase of BUN at 48 h were statistically statistical differences between groups A,B,C,D(3.21 ± 1.73 vs 0.12 ± 0.03;3.21 ± 1.73 vs 0.02 ± 0.17;3.21 ± 1.73 vs 1.04 ± 2.15;3.21 ± 1.73 vs 1.06 ± 0.68;p < 0.05).(3)Pathological changes of renal tubules: Morphologically,there was a statistically significant difference between the group E and the groups A,B,C,D(2.21±0.3 vs 0.09±0.12;2.21±0.3 vs 0.2±0.24;2.21 ± 0.3 vs 1.63 ± 0.54;2.21 ± 0.3 vs 0.96 ± 0.61;P < 0.05).In the apoptosis,a large number of renal tubular apoptosis cells were observed in the group E,and the apoptotic index was statistically different from the groups A,B,C,D(23.81±4.71 vs 1.37±1.26;23.81±4.71 vs 2.21±1.13;23.81±4.71 vs 15.19±2.28;23.81±4.71 vs 10.32±1.65;P<0.05).The expression of caspase-3 protein was the highest in group E,and there were statistical differences between group A,B,C and D(15.83±1.11 vs 2.48±0.84;15.83±1.11 vs 4.02±0.92;15.83±1.11 vs 11.68±1.87;15.83±1.11 vs 7.86±1.13;P<0.05).The expression of caspase-9 protein was the highest in group E,and there were statistical differences between group A,B,C and D(14.38±1.28 vs 2.91±0.95;14.38±1.28 vs 3.77±0.96;14.38±1.28 vs 12.12±2.5;14.38±1.28 vs 7.91±1.17;P<0.05).Conclusions:The classical + dehydration model group(group E)had a highest success rate,the highest increase in creatinine and urea nitrogen,the most significant pathological changes,and the obvious change of renal tissue oxidative stress index,which was the best Mouse model of contrast-induced acute kidney injury. |
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