| Background: Benign prostatic hyperplasia(BPH)is the most common degenerative disease in middle-aged and elderly men that affects their reproductive organs.Excessive alcohol can cause various reproductive diseases.How to prevent and treat reproductive damage caused by BPH and drinking alcohol has clinical significance.Dendrobium officinale polysaccharides(DOP)have many functions as antioxidant and anti-aging.However,it has not been reported whether DOP has therapeutic effect on BPH and reproductive damage.Objective: To establish a pathological model of prostatic hyperplasia in mouse,explore the reproductive damage of androgen and alcohol on the prostate and testis in mouse,and compare the therapeutic effects of DOP in different ways and duration on the BPH and testicular damage in the model mouse.Preliminary study on the mechanism of DOP in the treatment of benign prostatic hyperplasia,provide reference for clinical treatment of BPH.Methods:(1)(1)A mouse model of BPH induced by testosterone propionate(TP)was established.6 weeks mice were randomly divided into 9 groups with 15 in each group as following,control groups(NC7d,NC14 d,NC35d),testosterone propionate model group(TP7d,TP14 d,TP35d)and testosterone propionate + Dendrobium officinale polysaccharide(200mg/kg)group(TP+DOP7d,TP+DOP14d,TP+DOP35d).Group NC was subcutaneously injected with TP(10mg/kg)solution soybean oil every day,and TP and TP+DOP groups were subcutaneously injected with TP every day,and at the same time administration DOP or distilled water intragastrically.(2)Alcohol(AL)was used to establish a mouse reproductive injury model.6 weeks mice were randomly divided into 9 groups with 15 mice in each group as following,control group(NC7d,NC14 d,NC35d),alcohol model group(AL7d,AL14 d,AL35d)and alcohol + Dendrobium officinale polysaccharide(200mg/kg)group(AL+DOP7d,AL+DOP14d,AL+DOP35d),Group NC was given distilled water by gavage administration daily,group AL and AL+DOP were given alcohol(AL,50% alcohol,1.75ml/kg)intragastrically daily,and DOP or distilledwater was administered intragastrically after 2h.The mice were treated for 7d,14 d,and 35 d,after the last administration,all mice were killed and weighed,calculating the prostate and testis coefficient,meanwhile,observing the histopathological changes and detecting the sperm parameters,and also determining the lipid peroxidation and antioxidant content in the testis and prostate.(2)A mouse model of BPH induced by testosterone propionate(TP)and alcohol(AL)was established.6 weeks old mice were randomly divided into 9 groups with 15 in each group as following,control group(NC7d,NC14 d,NC35d),TP+AL model group(AL+TP7d,AL+TP14d,AL+TP35d)and TP+AL+DOP group(AL+TP+DOP7d,AL+TP+DOP14d,AL+TP+DOP35d),group NC was subcutaneously injected with soybean oil(the TP vehicle),and the distilled water was administered intragastrically after 1 hour everyday;TP+AL and AL+TP+DOP groups were subcutaneously injected with TP(10mg/kg)daily and were intragastrically administered with AL(50% alcohol,1.75ml/kg)after 1 hour,then after 2 hours of AL treatment,DOP or distilled water was intragastrically administered.The mice were treated with 7d,14 d,and 35 d,after the last treatment,all mice were killed and weighed,calculating the prostate and testicular index.In addition to sperm parameters and detection of antioxidant capacity of prostate and testicular tissues,the expression of corticosteroid related genes StAR and P450 scc mRNA in mice testis and the mRNA expression of 5-alpha reductase2 in the prostate were also detected by real time fluorescence quantitative PCR.Results:(1)In the mouse model of TP-induced BPH,compared with the control group,the antioxidant capacity in mouse testis and prostate tissues in the TP group were decreased,but there was no obvious change in the sperm quality and the histopathology of the prostate and testis in the TP7 d group,while the number and quality of spermatozoa in group TP14 d and 35 d were decreased.Hispathological observation found typical BPH and testicular damage.In the mouse model of AL-induced reproductive damage in mouse,the sperm quality was declined and the quantity was reduced,meanwhile the tissue antioxidant capacity in group AL(7/14/35d)were similar to the TP14 d and 35 d,but there is no hyperplasia inprostate.Compared with the TP or AL group,the DOP treatment group decreased the pathological damage of the prostate and(or)testis,and the quality and quantity of sperm were increased,as well as the antioxidant capacity of the prostate and testis,and the longer the DOP treatment time,the more effective the treatment was.(2)In the mouse model of TP and AL co-administration,compared with the control group,the antioxidant capacity of the testis and prostate and the quantity and quality of the sperm were reduced in AL+TP7d,14 d and 35 d groups,and histopathological observation revealed that there were typical hyperplasia in prostate,accompanied by severe hispathological damage of testis,which were worse by the time.Compared with the TP model group and the AL model group,the short-term of7 d can cause a typical BPH.Compared with the TP+AL group,the damage of the testis in the AL+TP+DOP group was reduced,the epithelial cell layers of the prostate were reduced,with the hyperplasia of the prostate reduced.The quality and quantity of sperm and the content of antioxidant enzymes in the prostate and testis were increased,and the effect of DOP on the treatment was better.Conclusion:(1)TP induced BPH mouse model did not affect mouse sperm quality in short term 14 d,and it did not cause BPH in 35 days after alcohol treatment.(2)Without TP as an inducement of BPH,drinking will not cause hyperplasia of prostate in mice.When TP causes BPH to cause androgen disorder in mice,alcohol drinking promotes the progression of prostatic hyperplasia,and the co-administration can cause typical BPH and obvious testicular tissue damage in the short term 7d.(3)The effect of DOP on BPH mice improves the antioxidant capacity of the prostate and testis,and regulates the expression of related genes,and there is a time effect. |
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