| Objective:Oral cancer is the most common oral malignant tumor,mainly squamous cell carcinoma,which greatly affects people’s health and quality of life.Its incidence has increased significantly in the past few decades and has attracted widespread attention worldwide.At present,traditional chemotherapy is still one of the main clinical anti-cancer methods,but its non-specific treatments often cause serious toxicity problems and life-threatening side effects.Therefore,drugs with biocompatibility and accurate drug delivery are urgently needed to overcome the limitations of traditional chemotherapyWith the development of materials science and biological science,metal organic frameworks(MOFs)has gradually come into the sight of scientific researchers.Metal organic frameworks is an organic-inorganic hybrid porous material with adjustable pore size,high load capacity,good biocompatibility and degradability,and has become an effective drug carrier in biomedicine.We adjusted the size of metal-organic framework materials to the nanometer level to form nanoscale metal-organic framework materials(NMOFs),which not only possessed the special physical and chemical properties of nanomaterials,but also possessed the characteristics of MOFs.NMOFs has become a better drug carrier material in all aspects.We combined doxorubicin(DOX)which is the broad-spectrum anti-cancer drug with NMOFs materials to form a nano-drug loading system to study its role in oral squamous cell carcinomaIn this study,two NMOFs materials MIL-101-NH2 and UIO-66-NH2 and their drug loading systems MIL-101-NH2-DOX and UIO-66-NH2-DOX were respectively studied in the preparation,morphological structure,drug release behavior,cytotoxicity,induction of apoptosis and cell uptake,so as to obtain a nanomaterials more suitable for clinical useMethods:1.Prepare nanomaterials MIL-101-NH2,UIO-66-NH2 by solvothermal method and use physical adsorption method to add DOX to the material to form drug loading system MIL-101-NH2-DOX,UIO-66-NH2-DOX2.The nanomaterials MIL-101-NH2,UIO-66-NH2 and drug-loading systems MIL-101-NH2-DOX,UIO-66-NH2-DOX were subjected to chemical characterization and structural inspection through electron microscopy,Fourier transform infrared Spectrum,electric potential and drug release behavior3.Detect the cytotoxicity of drug-loading systems MIL-101-NH2-DOX,UIO-66-NH2-DOX and simple DOX by CCK-8 method;detect the apoptosis of MIL-101-NH2,UIO-66-NH2,MIL-101-NH2-DOX,UIO-66-NH2-DOX,and simple DOX by flow cytometry;detect the cell uptake of MIL-101-NH2,UIO-66-NH2,MIL-101-NH2-DOX,UIO-66-NH2-DOX,and simple DOX by laser confocal microscopeResults:1.The prepared nanomaterial MIL-101-NH2 is a dodecahedron with a size of about 200nm;UIO-66-NH2 is an octahedron with a size of about 80nm.After drug loading,the potential of nanomaterials increased,indicating that MOFs were successfully loaded.The drug loading capacity of MIL-101-NH2 is 103mg/g,while that of UIO-66-NH2 is 76mg/g,and the drug release of MIL-101-NH2 is slower2.The cytotoxicity results showed that the cell survival rate of MIL-101-NH2-DOX was as low as 58.74%at the lowest concentration of 0.78μg/mL,while UIO-66-NH2-DOX and pure DOX were 79.10%and 75.42%MIL-101-NH2-DOX has the highest cytotoxicity at low concentration3.The apoptosis rates of pure DOX,drug-carrying systems UIO-66-NH2-DOX and MIL-1O1-NH2-DOX were 20.23%,15.21%,34.98%respectively MIL-101-NH2-DOX induced more apoptosis4.Materials and anticancer drugs in the prepared drug-loading systems MIL-101-NH2-DOX and UIO-66-NH2-DOX can enter cells together.Most anti cancer drugs DOX enter the nucleus,and some anti cancer drugs and materials accumulate in the cytoplasm;and nanomaterial MIL-101-NH2 can transport more drugs into cells than UIO-66-NH2Conclusion:The prepared nanomaterials have uniform size and stable structure MIL-101-NH2 has greater drug loading capacity and better sustained-release effect than UIO-66-NH2;at low concentration the drug loading system MIL-101-NH2-DOX has higher cytotoxicity than UIO-66-NH2-DOX and DOX;MIL-101-NH2-DOX can induce the apoptosis of more TCA8113 cells and carry more drugs into the cells.It has good application potential. |
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