Role Of TXNIP On Autopathy In Mouse Retinal Müller Glia Under Hyperglycemia Conditions And Its Mechanisms

Objective Diabetic retinopathy,diabetic retinopathy,DR)is a kind of vascular complications of diabetes is the most common,also is a kind of progressive neural degenerative diseases,the most main is the world’s adult population is one of the most serious blinding eye disease.To investigate the molecular mechanism of autophagy of Müllerr cells induced by thioredoxin interacting protein(TXNIP)during DR development.Methods Müller cells were high-glucose cultured and knockdown TXNIP using RNAi,then investigated the autophagy associated proteins and AKT/mTOR signaling pathway using immunofluorescence,Western blot and realtime PCR.Results Immune cell fluorescence tip under the condition of high glucose,TXNIP expression significantly increased,and the expression of GS were decreased(p < 0.01);And break the low group TXNIP TXNIP expression decreased significantly,while the expression of GS increased(p < 0.01);By Western blot and Real-time PCR results indicate the Müller cells induced by high sugar and low oxygen TXNIP,LC3 II,p62 / SQSTMl expression were significantly increased(P < 0.05);And after will knock TXNIP low Müller cells autophagy related proteins characteristic(LC3 Ⅱ,P62)expression was significantly reduced(P < 0.05);And p-Müller cells induced by high sugar Akt,p-mTOR protein expression levels showed a trend of rise significantly(p <0.01);On low TXNIP expression after p-Akt,p-mToR protein expression levels have corresponding lower(p < 0.05).Conclusion TXNIP may inhibition Müller cells autography and apoptosis via AKT/mTOR pathway in the early stage of DR.Therefore,inhibition of Müller TXNIP high expression in cells may be a hot spot t of DR and related vascular lesions in the future,and the further study of TXNIP of autophagy and the related mechanism maybe become a target of the research of DR.