Regulation Of Histone Modification On Gene Expression In GM12878 Cell Line And K562 Cell Line

The basics recent years,the occurrence of cancer is a serious threat to human life and health.With the development of high-throughput sequencing technology,a large amount of data including epigenetic modification have been obtained.It has been found that abnormal changes in epigenetic modification also play an important role in the regulation of cancer.DNA methylation and histone modification have made gratifying achievements in the study of blood cancer.Therefore,using bioinformatics to systematically analyze the regulation of epigenetic modification on cancer has become a research hotspot in bioinformatics.In the first mock exam,we selected 11 histone modifications,which were commonly used in the GM12878 cell line and K562 cell line,to study the effect of histone modification on gene expression.We also selected clinical data from acute myeloid leukemia(AML)and screened similar functional or functional genes through the weighted co-expression network to the same module.The main research contents of this paper are as follows:1.Using 11 kinds of histone modification data of the GM12878 cell line and K562 cell line,the histone modification signal was calculated in the range of 4000 bp upstream and downstream with TSS as the center,and the relationship between histone modification level and the gene expression level was analyzed by histone modification signal distribution.Furthermore,the correlation between histone modification in the K562 and the GM12878 cell lines was measured by the Spearman coefficient.It was found that some histone modification levels and gene expression were inhibited or promoted to some extent.At the same time,it was found that some histone modifications were highly correlated with each other,and there were multiple relationships regulating gene expression levels.2.In the gm12878 cell line and the K562 cell line,the differential expression genes were screened based on the adjusted P < 0.01 and log2(Fold Change)|> 1.By combining the differentially expressed genes with clinical data and samples of AML,we cluster the genes with similar biological functions or functions using a weighted co-expression network and get 8 modules.3.Gene annotation analysis is carried out for the genes contained in the module,and important gene clusters in the protein-protein interaction network(PPI)are searched through MCODE.The results showed that 15 genes were found,namely Blue module: P2RY10,GNG2,CYSLTR2;Brown module: CCR6,CXCL2,S1PR4;yellow module: ASB15,ZNF606,ZNF311;Green module IFNA21,IFNA8,IFNA2,LMO7,FBXO2,HERC5.It plays a role in protease degradation of ubiquitination and regulation of protein phosphorylation.