Study On The Chemcial Consitituents And Hypoglycemic Activity Of Selaginella Pulvinata

Selaginella pulvinata（Selaginella）,recorded in the Chinese Pharmacopoeia（2015 Edition）as“Juanbai”,widely distributed in sichuan,yunnan,tibet,guangxi,guangdong,jiangxi,hubei,henan,hebei and so on.It has the effects of promoting blood circulation,anti-inflammatory and heat dissipation.Studies reported that S.pulvinata is in rich flavonoids,polyphenols,lignans.Pharmacology research indicated S.pulvinata possess different acitivities including hypoglycemic,anti-oxidation,anti-aging,anti-tumor,anti-viral and so on.S.Pulvinata has great potential for development in hypoglycemic.In order to further develop medicinal value and utilize chinese medicine resource,we studied the phytochemcials and hypoglycemic activity of S.pulvinata.Different chromatographic methods including MCI chromatography,C₁₈column chromatography,silica gel chromatography,sephadex LH-20 chromatography,MPLC and prep-HPLC were used to study the phytochemcials of S.pulvinata.As a result,twenty compounds were isolated from 95%ethanol extract from S.pulvinata.Their structures were identified by comprehensive using of spectrum technologies（UV,IR,NMR,CD）and chemical methods as follows:tamariscinoside G（SP-1*）,tamariscinoside H（SP-2*）,pulvin A（SP-3*）,moellenosideC（SP-4*）,tamariscinolV（SP-5）,（2R,3S）-dihydro-2-（3,5-dimethoxy-4-hydroxy--phenyl）-7-methoxy-5-acetylbenzofura n（SP-6）,syringaresinol（SP-7）,pulvpeptin A（SP-8*）,selaginellin（SP-9）,selaginpulvilin A（SP-10）,3-（4-hydroxy-phenyl）-6,7-dihydroxy coumarin（SP-11）,amentoflavone（SP-12）,（SP-13）,unciflavone E（SP-14）,syringic acid（SP-15）,2,6-dihydroxy-3,4-dim ethylbenzoic acid methyl ester（SP-16）,4-ethoxy benzoic acid（SP-17）,phenylalanine（SP-18）,loliolide（SP-19）,trehalose（SP-20）.Among them,SP-1～SP-4 and SP-8 were determined to be new compounds,SP-1～4 were belonged to the norlignans,SP-8 was a cyclic peptide containing eight amino acid residues and also isolated from Selaginella for the first time.SP-1～SP-2 and SP-5 were isolated as a pair of epimers,but they were difficult to separate in conventional C₁₈prep-HPLC.They were successfully separated by a Daicel Chiralpak AD-H type chiral column to get SP-1a～SP-1b,SP-2a～SP-2b and SP-5a～SP-5b.SP-13 and SP-16～19 were isolated from Selaginella for the first time.The hypoglycemic activities of the isolated were evaluated in 3T3-L1 cell,the inhibition ofα-glucosidase experiment were also tested in this thesis.The effect of stimulating glucose uptake in 3T3-L1 adipocytes showed that SP-1,SP-3～4 and SP-12 could increase glucose uptake in 3T3-L1 cells in different degrees at 10μg/m L.Among them,SP-3 and SP-4 showed concentration dependence in promoting glucose uptake in 3T3-L1 adipocytes.The results ofα-glucosidase experiment indicated none of the tested compounds showed obvious inhibiton activities at 5 mg/mL.