Application Of Mesoporous Biofilm Chromatography In Screening Of Active Components In Natural Medicinal Herbs

Drug screening is a key step in the development process of new drugs.It is an effective method for obtaining target components from natural drugs or synthetic compounds by using appropriate screening methods and screening techniques.The classification of drug screening includes various aspects such as pharmacological effects,compound structure,and biological activity.Through effective drug screening,the potential target ingredients and the process of new drug development can be studied.There are various problems in traditional drug screening methods.For example,the price of animal screening is expensive,and the pathological site and drug mechanism of animal screening are unknown.Cell membrane chromatography requires highly screening technology and cells are susceptible to inactivation.Liposome chromatography lacks receptors,rendering it not suitable for studying the interactions between drug and membrane receptors.Therefore,it's the focus of recent research to find an efficient and rapid method to screen potential drugs.In order to solve the problems in the existing technology,this thesis studied a mesoporous biofilm chromatography method based on target protein.It combined biofilm chromatography with receptor chromatography.Combined with HPLC,this stationary phase can be used to evaluate the interaction of drugs-receptors and eliminate the interference of non-acting impurities.The chromatographic column can be reused many times,and the preparation process is simple.Specific research is as follows :Firstly,Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol)(P123),potassium chloride(Kcl),Tetraethyl orthosilicate(TEOS),1,3,5-Trimethylbenzene(TMB)were used as raw materials to prepare mesoporous silicon materials.Mesoporous materials with controlled scale were prepared by adjusting the ratio of P123 and TMB,changing the time of hydrothermal crystallization,the temperature of hydrothermal crystallization,and stirring conditions.Secondly,mesoporous silicon materials were prepared based on the first step.And the ?-glucosidase receptor was loaded through the method of ice bath.Mesoporous Biofilm Chromatography(MBC)stationary phase based on ?-glucosidase receptor was prepared by inverse evaporation method using soybean lecithin(PC)and cholesterol as raw materials.The ratio of P123 to TMB,the ratio of soybean lecithin and cholesterol,the adsorption time of ?-glucosidase receptor,the p H value of the adsorption solution,and the maximum load were optimized to determine the best preparation plan.The obtained materials were characterized by Fourier infrared spectroscopy(FT-IR),scanning electron microscope(SEM)and energy dispersive spectrometer(EDS).Thirdly,by changing the column temperature,mobile phase flow rate and p H,miglitol and acarbose were used to study the difference in retention behavior of ?-glucosidase inhibitors on MBC stationary phase and blank biofilm chromatography stationary phase.The non-?-glucosidase inhibitor was used as a negative control to verify the screening effect of MBC on ?-glucosidase inhibitor.Fourthly,the inhibitory ability of active ingredients in different natural medicinal herbs was studied.Schisandra chinensis and Terminalia chebula Retz were selected as experimental objects.Finally,the active ingredients had been successfully screened from Schisandra chinensis and Terminalia chebula Retz.This work demonstrated the feasibility of MBC in the screening of active ingredients of natural medicinal herbs.