Experimental Study Of Dihydroartemisin Promoting Osteogenic Differentiation Of Bone Marrow Mesenchymal Stem Cells

Dihydroartemisinin(DHA),which is considered to be one of the active compounds within Artemisia annua,has extensively been used in recent years as the most effective drug against malaria,having many biological functions including anticancer,antifungal and immunomodulatory activities.However,whether DHA plays a role in the regulation of the proliferation and osteogenic differentiation of human mesenchymal stem cells(h MSCs)remains unknown.We explored DHA's effect on h MSCs' proliferation as well as the osteogenic differentiation,together with its underlying mechanisms of action.Research purposes : to study the effect and mechanism of dihydroartemisinin on osteogenic differentiation of HMSCs.Research methods:Using experiments in vitro: Western Blot,QT-PCR,immunofluorescence,inhibitor experiments,alizarin red staining and CCK-8 experiments to study the effect of dihydroartemisinin on osteogenic differentiation of stem cells.Conclusion:This is the first study to elucidate the effect of DHA on HMSCs osteogenic differentiation.Our study showed that DHA had no significant effect on HMSCs proliferation at low concentrations,but could promote HMSCs osteogenic differentiation by activating ERK1/2 and Wnt/? signaling pathways.Hence,DHA is likely to be a potential treatment for accelerating fracture healing.