The Effect And Mechanism Of BAORUJI Optimizers In Mongolian Medicine On Preventing Ulcerative Colitis Associated Cancer

Objective:by observing the Mongolian medicine treasure as the agent of methane oxidation azo(AOM)/dextran sodium sulfate(DSS)induced ulcerative colitis associated colorectal cancer type(UCACC)mice endothelial nitric oxide synthase(eNOS),carbon monoxide(CO),urinary sulfur ether beta synthetase(CBS)content,the influence of the gas signal molecule as a whole system and the influence of Wnt pathways beta catenin protein,to study the effect of Mongolian medicine such as agent for UCACC treasure and some chemical prevention mechanism.Guided by the holistic view of Mongolian medicine,it provides experimental basis for clinical research on dialectical medication and niruha treatment with rich experience,good curative effect and low medical cost.Methods:A total of 144 SPF Balb/C male mice were randomly divided into the blank group,model group,baoru agent low,medium and high dose treatment group and mesalazine control group,with 24 mice in each group.Except the normal group,the rest groups were prepared with the combined method of AOM/DSS.Starting from the 5th day of the experiment,the low-dose,medium-dose and high-dose baoru agent groups were given the corresponding dose of niruha treatment,and the control group was given mesalazine enema.At day 28 and 70,10 mice in each group were randomly treated.Serum eNOS,CO,and CBS levels were determined by Elisa and the expression of-catenin protein was determined by IHC staining.Results:1.The changes of eNOS in the serum of ucacc model mice during tumor formation and tumor developmentIn tumor formation period,there was significant difference between the blank group and the model group(P<0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was significant difference between the model group and the middle dose group(P<0.05).There was significant difference between the model group and the high-dose group(P<0.05).There were significant differences between model group and mesalazine group(P<0.05).There was no significant difference between the low-dose group and the middle-dose group(p>0.05).There were significant differences between the low-dose group and the high-dose group(P<0.05).There was significant difference between the low dose group and mesalazine group(P<0.05).There was significant difference between the middle dose group and the high dose group(P<0.05).There was significant difference between the middle dose group and mesalazine group(P<0.05).There was no significant difference between the high dose group and the mesalazine group(p>0.05).In tumor development period,there was significant difference between the blank group and the model group(P<0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was significant difference between the model group and the middle dose group(P<0.05).There was significant difference between the model group and the high-dose group(P<0.05).There were significant differences between model group and mesalazine group(P<0.05).There was no significant difference between the low-dose group and the middle-dose group(p>0.05).There were significant differences between the low-dose group and the high-dose group(P<0.05).There was significant difference between the low dose group and mesalazine group(P<0.05).There was significant difference between the middle dose group and the high dose group(P<0.05).There was significant difference between the middle dose group and mesalazine group(P<0.05).There was no significant difference between the high dose group and the mesalazine group(p>0.05).2.The changes of CO content in serum of ucacc model mice during tumor formation and tumor developmentIn tumor formation period,there was no significant difference between the blank group and the model group(p>0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was no significant difference between the model group and the middle dose group(p>0.05).There was no significant difference between the model group and the high-dose group(p>0.05).There was no significant difference between model group and mesalazine group(p>0.05).There was no significant difference between the low-dose group and the middle-dose group(p>0.05).There was no significant difference between the low dose group and the high dose group(p>0.05).There was no significant difference between the low dose group and the mesalazine group(p>0.05).There was no significant difference between the middle-dose group and the high-dose group(p>0.05).There was no significant difference between the middle dose group and mesalazine group(p>0.05).There was no significant difference between the high dose group and the mesalazine group(p>0.05).In tumor development period,there was significant difference between the blank group and the model group(P<0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was significant difference between the model group and the middle dose group(P<0.05).There was significant difference between the model group and the high-dose group(P<0.05).There were significant differences between model group and mesalazine group(P<0.05).There was no significant difference between the low-dose group and the middle-dose group(p>0.05).There were significant differences between the low-dose group and the high-dose group(P<0.05).There was significant difference between the low dose group and mesalazine group(P<0.05).There was significant difference between the middle dose group and the high dose group(P<0.05).There was significant difference between the middle dose group and mesalazine group(P<0.05).There was significant difference between the high dose group and the mesalazine group(P<0.05).3.The change of CBS content in the serum of ucacc model mice during tumor formation and tumor developmentIn tumor formation period,there was no significant difference between the blank group and the model group(p>0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was no significant difference between the model group and the middle dose group(p>0.05).There was no significant difference between the model group and the high-dose group(p>0.05).There was no significant difference between model group and mesalazine group(p>0.05).There was no significant difference between the low-dose group and the middle-dose group(p>0.05).There was no significant difference between the low dose group and the high dose group(p>0.05).There was no significant difference between the low dose group and the mesalazine group(p>0.05).There was no significant difference between the middle-dose group and the high-dose group(p>0.05).There was no significant difference between the middle dose group and mesalazine group(p>0.05).There was no significant difference between the high dose group and the mesalazine group(p>0.05).In tumor development period,there was significant difference between the blank group and the model group(P<0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was significant difference between the model group and the middle dose group(P<0.05).There was significant difference between the model group and the high-dose group(P<0.05).There were significant differences between model group and mesalazine group(P<0.05).There were significant differences between the low-dose group and the middle-dose group(P<0.05).There were significant differences between the low-dose group and the high-dose group(P<0.05).There was significant difference between the low dose group and mesalazine group(P<0.05).There was significant difference between the middle dose group and the high dose group(P<0.05).There was significant difference between the middle dose group and mesalazine group(P<0.05).There was significant difference between the high dose group and the mesalazine group(P<0.05).4.The changes of eNOS,CO and CBS in the serum of ucacc model mice during tumor formation and tumor developmentThe levels of eNOS,CO and CBS in the serum of ucacc model were significantly higher than those in the tumor formation stage(P<0.5).5.Expression of ?-Catenin in the colon of UCACC model mice during tumor formation and tumor developmentIn tumor formation period,there was significant difference between the blank group and the model group in tumor formation period(P<0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was significant difference between the model group and the middle dose group(P<0.05).There was significant difference between the model group and the high-dose group(P<0.05).There were significant differences between model group and mesalazine group(P<0.05).There was no significant difference between the low-dose group and the middle-dose group(p>0.05).There was no significant difference between the low dose group and the high dose group(p>0.05).There was no significant difference between the low dose group and the mesalazine group(p>0.05).There was significant difference between the middle dose group and the high dose group(P<0.05).There was no significant difference between the middle dose group and mesalazine group(p>0.05).There was no significant difference between the high dose group and the mesalazine group(p>0.05).In tumor development period,there was significant difference between the blank group and the model group(P<0.05).There was significant difference between the model group and the low-dose group(P<0.05).There was significant difference between the model group and the middle dose group(P<0.05).There was no significant difference between the model group and the high-dose group(p>0.05).There were significant differences between model group and mesalazine group(P<0.05).There was no significant difference between the low-dose group and the middle-dose group(p>0.05).There was no significant difference between the low dose group and the high dose group(p>0.05).There was no significant difference between the low dose group and the mesalazine group(p>0.05).There was significant difference between the middle dose group and the high dose group(P<0.05).There was no significant difference between the middle dose group and mesalazine group(p>0.05).There was no significant difference between the high dose group and the mesalazine group(p>0.05).Conclusion:1.Mongolian medicine BAORU can regulate the content of eNOS,CO and CBS in tumor formation and development of UCACC model mice.2.Mongolian medicine BAORU can regulate the expression of ?-Catenin in Wnt pathway during tumor formation and tumor development in ucacc model mice.3.Mengyibaoru can regulate the gas signal molecules and Wnt/?-Catenin in ucacc model mice,thus play a role in tumor formation and development.Gas signaling molecular system plays a regulatory role in the activation of Wnt/?-Catenin pathway and then participates in the formation and development of tumor.Gas signaling molecular system is the activator of Wnt/?-Catenin pathway.4.Guided by the basic theory of Mongolian medicine,through the observation of the action mechanism of Baoru agent of Mongolian medicine on ucacc mice,it is considered that the gas signal molecule is part of the material basis of Hey i.