Expression And Significance Of Beclin-1 And LC3B In Neuroblastoma

Objective: This study aimed to detect the expression of LC3 B and Beclin-1 in the neuroblastoma and normal lymph node tissues,and analyzed the differences of LC3 B and Beclin-1 between neuroblastoma and normal lymph node tissues,and analyzed the relationship between LC3 B and Beclin-1 expressions in neuroblastoma with the clinicopathological features of neuroblastoma in its sufferers.Methods: This study used data from 38 patients diagnosed with neuroblastoma and 16 patients' normal lymph nodes seen at The Fourth Hospital of Hebei Medical University between October 2010 and December 2018.The expression of LC3 B and Beclin-1 in specimens was detected by immunohistochemical staining.The relationship between the expression levels of LC3 B and Beclin-1 and clinical features was analyzed by Chi-square test or Fisher exact probability method.Spearman correlation analysis was used to explore the correlation between LC3 B and Beclin-1.P <0.05 was considered statistically significant.Results: 1.The expression of LC3 B was located in cytoplasm,and the expression of Beclin-1 was located in nucleus.The expression of LC3 B and Beclin-1 in neuroblastoma was significantly higher than that in normal lymph node tissues.2.The positive expression of LC3 B and Beclin-1 in neuroblastoma was related to pathological type and risk group.There was no significant difference in sex,age or tumor markers(NSE,LDH,VMA).3.Spearman correlation analysis showed that the expression of LC3 B and Beclin-1 were positively correlated in the neuroblastoma(r=0.447,P=0.006).Conclusions: 1.The expression of LC3 B and Beclin-1 in neuroblastoma is higher than that in normal tissus,and is related to the pathological type and risk group of the neuroblastoma.LC3 B and Beclin-1 may play a role in the development of the neuroblastoma.2.The expression of LC3 B and Beclin-1 are positively correlated in the neuroblastoma,Beclin-1 and LC3 B may play a synergistic role in autophagy process of neuroblastoma.3.The expression of LC3 B and Beclin-1 is related to the pathological type and risk group of the neuroblastoma,which may help to estimate the severity ang prognosis of the neuroblastoma.