| Background & Aims:The tumor microenvironment is an important influencing factor in the development of tumors.The myeloid-derived suppressor cells MDSCs are an important part of the tumor microenvironment.MDSCs and related inflammatory factors interact with chemokines and their receptors,and they play a role in tumor development.A vital role.In recent years,MDSCs have been shown to play a role in the progression and metastasis of various tumors.However,the expression of MDSCs in early gastric cancer and its recruitment mechanism are still unclear.This paper intends to study the expression level of MDSCs in peripheral blood and tissues of patients with early gastric cancer and the possible related mechanisms.Preliminary explore its significance in the occurrence and development of gastric cancer.Methods:The amount of MDSCs in the peripheral blood of healthy subjects,early gastric cancer patients and patients with advanced gastric cancer was detected by flow cytometry,and the differences in the numbers of MDSCs between the groups were compared.By collecting gastric biopsy tissues from healthy subjects and gastric cancer tissues from patients with gastric cancer Immunohistochemical staining was used to compare the number of MDSCs and the expression of chemokine receptor CXCR2 in adjacent tissues,metastatic lymph nodes,and metastatic lymph nodes.Clinical data such as age,gender,tumor site,tumor size,pathological type,depth of invasion,and lymph node metastasis were recorded,the number of MDSCs and the expression of CXCR2 in each tissue were statistically analyzed,and the number of MDSCs in the progression of gastric cancer was analyzed.Changes and their possible related mechanisms of recruitment in gastric cancer were statistically analyzed.Results:The results of flow cytometry showed that the number of MDSCs in peripheral blood of patients with early gastric cancer and patients with advanced gastric cancer were significantly higher than that of healthy people,but the number of MDSCs in peripheral blood of patients with advanced gastric cancer was not significantly different from that of patients with early gastric cancer.Immunohistochemical results showed that the number of MDSCs and the expression level of CXCR2 in early gastric cancer tissue and advanced gastric cancer tissue were significantly higher than those in the corresponding paracancerous tissue and gastric biopsy tissues of healthy people;the number of MDSCs and the expression level of CXCR2 in metastatic lymph nodes both were significantly higher than those lymph nodes without metastasis.However,the number of MDSCs and the expression level of CXCR2 in advanced gastric cancer tissues were not significantly different from those in early gastric cancer tissues.The results of immunohistochemistry showed that there was colocalization of CD11 b and CXCR2,and the expression of the two was positively correlated.Conclusion:MDSCs are increased in peripheral blood and tissues of patients with early gastric cancer.Gastric cancer cells may recruit CXCR2 + MDSCs to tumor tissues through the CXCLs/CXCR2 axis,eventually promoting the progression and metastasis of gastric cancer. |
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