PIWI-interacting RNA 000291 And 002372 In The Development Of Colorectal Cancer

Objective: To detect the expression levels of PIWI-interacting RNA000291(piRNA000291)and PIWI-interacting RNA002372(piRNA002372)in colorectal cancer tissues,paracancer normal tissues and precancerous lesions,as well as the roles of these two piRNAs in the development of colorectal cancer.Method: 1.To collect the Inner Mongolia medical university people's hospital affiliated gastroscope room's colorectal specimens of high-grade intraepithelial neoplasia and gastrointestinal surgery in the treatment of patients with colorectal cancer radical specimens of colorectal cancer tissue and normal tissue adjacent to carcinoma specimens.Using real-time fluorescent quantitative PCR(real-time fluorescence PCR,policy RT-q PCR)to detect piRNA000291 and piRNA002372 in colorectal cancer tissues and normal tissues adjacent to carcinoma and precancerous lesions in the expression level of organization,and its correlation with clinical pathological parameters;2.Two piRNAs sequence specific antagonists were transfected into colon cancer cell line HT29,and changes in the proliferation capacity of HT29 cells were detected using Incu Cyte S3 dynamic cell imaging analysis system;3.RT-q PCR detection of piRNA000291 and piRNA002372 expression in normal colon epithelial cells,colon cancer cell-derived exosomes.Results: 1.The relative expression of piRNA000291 in colorectal cancer tissues(1.325,0.305-161.233)was significantly higher than that in para-carcinoma normal tissues(0.618,0.009-40.899)(2.144 times,P=0.001),and significantly higher than that in high-grade intraepithelial neoplasia tissues(0.478,0.298-16.382)(2.772 times,P=0.023).There was no significant difference in the relative expression of piRNA000291 between high-grade intraepithelial neoplasia and paracancer normal tissues(0.773 times,P=0.857).The relative expression of piRNA002372 in colorectal cancer tissues(0.017,0.004-2.253)was significantly higher than that in para-carcinoma normal tissues(0.011,0.001-0.275)(1.545 times,P=0.026),and significantly higher than that in high-grade intraepithelial neoplasia tissues(0.007,0.003-0.092)(2.429 times,P=0.030).There was no significant difference in the relative expression of piRNA002372 in high-grade intraepithelial neoplasia and para-carcinoma normal tissues(0.636 times,P=0.157).There was no significant correlation between the relative expression of piRNA000291 in colorectal cancer tissues and its clinicopathological parameters: age(P=0.227),gender(P=0.651),lesion site(P=0.420),presence or absence of lymph node metastasis(P=0.091),tumor size(P=0.755),differentiation degree(P=0.308)and TNM stage(P>0.05).There was no significant correlation between the relative expression of piRNA002372 in colorectal cancer tissues and its clinicopathological parameters: age(P=0.787),sex(P=0.811),lesion site(P=0.762),presence or absence of lymph node metastasis(P=0.279),tumor size(P=0.985),differentiation degree(P=0.941)and TNM stage(P>0.05);2.piRNA000291 antagonist transfected into colon cancer cells could not inhibit cell proliferation;After transfection with piRNA002372 antagonist into colon cancer cells,the cell proliferation was inhibited;3.The expression of piRNA000291 in colon cancer cell-derived exosomes was significantly higher than that in colon normal epithelial cell-derived exosomes(11.369 times);piRNA002372 in colon cancer cell-derived exosomes was lower than that in colon normal epithelial cell-derived exosomes(0.018 times).Conclusion: the abnormally high expression of piRNA000291 and piRNA002372 is closely related to the development of colorectal cancer.High expression of piRNA002372 in colon cancer cells promotes the proliferation of cancer cells.piRNA000291 may be involved in the remodeling of the colon cancer micro-environment.